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NlaIII is a restriction endonuclease.

Mechanism

Recognition site of NlaIII with a red line indicating the cutting pattern

NlaIII recognizes the palindromic and complementary DNA sequence of CATG/GTAC and it cuts outside of the G-C base pairs. This cutting pattern results in sticky ends with GTAC overhangs at the 3' end.^[1]

Uses

NlaIII is used in molecular biology techinques and experimental procedures such as:^[2]

References

^ ^a ^b Jack J. Pasternak (14 June 2005). An Introduction to Human Molecular Genetics: Mechanisms of Inherited Diseases. John Wiley & Sons. ISBN 978-0-471-71917-5.
^ "Hin1II (NlaIII) (5 U/L) - Thermo Fisher Scientific". www.thermoscientificbio.com.

Category:Genetics Category:Molecular biology Category:Biotechnology Category:Restriction enzymes

[Pasternak2005-1] Jack J. Pasternak (14 June 2005). An Introduction to Human Molecular Genetics: Mechanisms of Inherited Diseases. John Wiley & Sons. ISBN 978-0-471-71917-5.

[2] "Hin1II (NlaIII) (5 U/L) - Thermo Fisher Scientific". www.thermoscientificbio.com.

[1]

[2]

v t e Restriction modification system: restriction enzyme
Basic Concept	Isoschizomer Neoschizomer Isocaudomer
Recognition sequence 4bp	TaqI Sau3A AluI* HaeIII* DpnII NlaIII HpaII
Recognition sequence 5bp	EcoRII FokI
Recognition sequence 6bp	AaaI BglII PovII* EcoRV* EcoRI BamHI HindIII XbaI XhoI SacI PstI NdeI
Recognition sequence 8bp	NotI
Lists	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv C-D E-F G-K L-N O-R S T-Z
* means cleavage produces blunt ends