NotI is a restriction enzyme isolated from the bacterium Nocardia otitidis.[1]

Recognition sequence

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Palindromic recognition sequence of NotI:[1]

5'-GCGGCCGC-3'
3'-CGCCGGCG-5'

The ends generated by NotI digest:

5'---GC     GGCCGC---3'
3'---CGCCGG     CG---5'

References

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  1. ^ a b Biolabs, New England. "NotI | NEB". www.neb.com. Retrieved 2016-05-08.