Intact antibodies can also be too large to dye cancer cells in vivo.[1] Their size results in slow tumor penetration and long circulating half life. Research has been done investigating the use of diabodies to get around this limitation.[2]
Outline for improvement of my article:
I want to add more about the background of Immunofluorescence mainly bridging the gap between immunohistochemistry and immunofluorescence. The current lead section mentions it as a subclass but does not make it clear how the two ideas are connected. I would also want to include a section called "Super Resolution Methods." Again, the lead paragraph suggests these methods but does not provide an adequate amount off depth in the subject. I ant to briefly go into the different super-resolution methods that can be used in relation to immunofluorescence. I also want to add information on the specific fluorophores and the chemistry behind their binding to antibodies used for fluorophores. This also includes going into more specific details about limitations surrounding immunofluorescence in certain applications in both the clinical setting and in research.
- ^ Sonn, Geoffrey A.; Behesnilian, Andrew S.; Jiang, Ziyue Karen; Zettlitz, Kirstin A.; Lepin, Eric J.; Bentolila, Laurent A.; Knowles, Scott M.; Lawrence, Daniel; Wu, Anna M. (2016-03-14). "Fluorescent Image–Guided Surgery with an Anti-Prostate Stem Cell Antigen (PSCA) Diabody Enables Targeted Resection of Mouse Prostate Cancer Xenografts in Real Time". Clinical Cancer Research. 22 (6): 1403–1412. doi:10.1158/1078-0432.ccr-15-0503.
- ^ Sonn, Geoffrey A.; Behesnilian, Andrew S.; Jiang, Ziyue Karen; Zettlitz, Kirstin A.; Lepin, Eric J.; Bentolila, Laurent A.; Knowles, Scott M.; Lawrence, Daniel; Wu, Anna M. (2016-03-14). "Fluorescent Image–Guided Surgery with an Anti-Prostate Stem Cell Antigen (PSCA) Diabody Enables Targeted Resection of Mouse Prostate Cancer Xenografts in Real Time". Clinical Cancer Research. 22 (6): 1403–1412. doi:10.1158/1078-0432.ccr-15-0503.