Peer Review - Marissa Ogata
Overall I think you did an excellent job with this page. I made a very minor grammatical error (added an apostrophe where necessary), but besides that I didn't see many grammatical - if not any - grammatical errors which is good. One thing I think would be important to include is what happens after the immunofluorescent test is complete? Is it possible to cleave the antibodies and the fluorophore back off of the molecule? And if so, what is the chemical mechanism by which this occurs? Also, when photobleaching is mentioned, there is a word-link to another wiki page for photobleaching. However, it would be helpful to give a very brief, one sentence explantation or definition of photobleaching rather than jumping into how to reduce photobleaching without explaining initially what it is. Also, I think the captions for the pictures could be shortened a bit; try to make it a bit more concise. If certain information in the caption is important, consider moving it from the caption into the main body/text where it is relevant.
Peer Review of Immunofluorescence by Kevin
The use of the personal pronoun you needs to be changed and modified in the section of Preparation of Fluoresence. In Primary Immunofluoresence, I think that the cost of the technique might not need to be mentioned. In the second and third paragraph of secondary immunofluoresence, there are no citation and an example is provided in paragraph 3. You might want to consider cutting paragraph 3 out and finding a source to support what is written in paragraph 2. In the third sentence of 1st paragraph of Limitations, you should consider another way to start the sentence because their is vague. The second cited source in bibliography should not be used, as it does not seem to be a reputable source. Overall, I think that the whole section flows very well, is organized clearly and distributed evenly, and the writing is clear.
My Article:
https://en.wikipedia.org/wiki/Immunofluorescence
Direction of edits:
I want to go into the limitations and advancements made in immunofluorescence. I want to add more detail about the method and how the antibodies are made for this technique to work.
Sample edit:
Intact antibodies can also be too large to dye cancer cells in vivo.[1] Their size results in slow tumor penetration and long circulating half life. Research has been done investigating the use of diabodies to get around this limitation.
My References:
| This user is a student editor in Wikipedia:Wiki_Ed/UCLA/Chem_153A_Honors_(Winter_2017). Student assignments should always be carried out using a course page set up by the instructor. It is usually best to develop assignments in your sandbox. After evaluation, the additions may go on to become a Wikipedia article or be published in an existing article. |
- ^ a b Sonn, Geoffrey A.; Behesnilian, Andrew S.; Jiang, Ziyue Karen; Zettlitz, Kirstin A.; Lepin, Eric J.; Bentolila, Laurent A.; Knowles, Scott M.; Lawrence, Daniel; Wu, Anna M. (2016-03-14). "Fluorescent Image–Guided Surgery with an Anti-Prostate Stem Cell Antigen (PSCA) Diabody Enables Targeted Resection of Mouse Prostate Cancer Xenografts in Real Time". Clinical Cancer Research. 22 (6): 1403–1412. doi:10.1158/1078-0432.ccr-15-0503.
- ^ Watkins,, Foster, John; M.,, Gillen, Kathy. Microbiology : an evolving science. ISBN 0393919295. OCLC 863158944.
{{cite book}}: Check|isbn=value: checksum (help)CS1 maint: extra punctuation (link) CS1 maint: multiple names: authors list (link) - ^ Reinig, Marc R.; Novak, Samuel W.; Tao, Xiaodong; Bentolila, Laurent A.; Roberts, Dustin G.; MacKenzie-Graham, Allan; Godshalk, Sirie E.; Raven, Mary A.; Knowles, David W. (2016-01-01). "Enhancing image quality in cleared tissue with adaptive optics". Journal of Biomedical Optics. 21 (12): 121508–121508. doi:10.1117/1.JBO.21.12.121508. ISSN 1083-3668.
- ^ Wei, Qingshan; Qi, Hangfei; Luo, Wei; Tseng, Derek; Ki, So Jung; Wan, Zhe; Göröcs, Zoltán; Bentolila, Laurent A.; Wu, Ting-Ting (2013-10-22). "Fluorescent Imaging of Single Nanoparticles and Viruses on a Smart Phone". ACS Nano. 7 (10): 9147–9155. doi:10.1021/nn4037706. ISSN 1936-0851. PMC 3951925. PMID 24016065.
{{cite journal}}: CS1 maint: PMC format (link) - ^ Mitchell-Jordan, Scherise; Chen, Haodong; Franklin, Sarah; Stefani, Enrico; Bentolila, Laurent A.; Vondriska, Thomas M. "Features of endogenous cardiomyocyte chromatin revealed by super‐resolution STED microscopy". Journal of Molecular and Cellular Cardiology. 53 (4): 552–558. doi:10.1016/j.yjmcc.2012.07.009. PMC 3704345. PMID 22846883.
{{cite journal}}: CS1 maint: PMC format (link) - ^ Rachmadi, Andri T.; Torrey, Jason R.; Kitajima, Masaaki (2016-11-15). "Human polyomavirus: Advantages and limitations as a human-specific viral marker in aquatic environments". Water Research. 105: 456–469. doi:10.1016/j.watres.2016.09.010. ISSN 1879-2448. PMID 27665433.