Erkad

Bacillus Thuringiensis

Hi Erkad. I was just wondering why you changed one of my Bacillus Thuringiensis edits. I thought it read clearer the way I had it. AIRcorn (talk) 06:15, 10 February 2010 (UTC) Hi AIRcorn. I found time for reply.Sorry for delay.First of all it is very strange to me ,that you "now is writing a report at the moment about Bt and other pesticides " have been use only Google Scholar as a source for evaluation of scholarly research contribution to the academic field for Zakharyan R.A. For this it was necessary also go to original articles published by prominent scientists in the field... Articles : Zakharyan R.A.et al. Possible role of extrachromosomal DNA in the formation of the isecticidal endotoxin of Bacillus thuringiensis. Inst.Exp. Bol., Yerevan,USSR. Doklady Akademii Nauk Armyanskoi SSR (1976), 63(1), 42-47. CODEN: DANAAW ISSN: 0321-1339.Journal written in Russian. CAN 86:68301 CAPLUS (Copyright 2003 ACS). Zakharyan R.A. "Study of plasmids and specific endonucleases of Bacillus thuringiensis". Genet. Actinomitsetov i Batsill. Sb. Dokl. Sovet.- American Konf., Erevan (1977), 249-252. From: Ref.Zh., Biol.Khim. 1979,Abstr. No. 17Kh102. Journal written in Russian. CAN 91: 207298 AN 1979:607298 CAPLUS (Copyright 2003 ACS) and Zakharyan R.A et al.(1979). "Plasmid DNA from Bacillus thuringiensis".(USSR) Microbiologiya 48 (2): 226–229. ISSN 0026-3656. became available in international circulation to the world scientific community already in 1976-79.See example of citations in next selected articles,writed by prominent scientists and listed below,: Clayton C. Beegle and Takhashi Yamamoto (1992).Review. "INVITATION PAPER ( C. P. ALEXANDER FUND) : History of Bacillus thuringiensis berliner research and development". Canadian Entomologist 124: 587–616. doi:10.4039/Ent124587-4. Dean D.H. (October 1984).Review."Biochemical Genetics of the Bacterial Insect-Control Agent Bacillus thuringiensis:Basic Principles and Prospects for Genetic Engineering". Biotechnology and Genetic Engineering Reviews-vol.2,341-363. XU Jian, LIU Qin, YIN Xiang-dong and ZHU Shu-de (2006). "A review of recent development of Bacillus thuringiensis ICP genetically engineered microbes". Entomological Journal of East China 15 (1): 53–58.. R. LANDEN, A. HEIERSON AND H. G. BOMAN, A Phage for Generalized Transduction in Bacillus thuringiensis and Mapping of Four Genes for Antibiotic Resistance .Journal of General Microbiology (198 l), 123,49-59. Printed in Great Britain . N. Shivarova 1 *, W. Förster 1 2, H.-E. Jacob 1 2, R. Grigorova 1 2 Microbiological implications of electric field effects VII. Stimulation of plasmid transformation of Bacillus cereus protoplasts by electric field pulses . Z Allg Mikrobiol.1983, v. 23(9),595-599. V.I. Miteva, N.I. Shivarova and R.T. Grigorova(1981) Transformation of Bacillus thuringiensis protoplasts by plasmid DNA. FEMS Microbiology Letters 12 (1981) 253-256 KAZUNORI ABE, ROBERT M. FAUST , LEE A. BULLA, JR.t ,"Plasmid Deoxyribonucleic Acid in Strains of Bacillus sphaericus and in Bacillus moritai’ "JOURNAL OF INVERTEBRATE PATHOLOGY 41,328-335(1983). Karabekov B.P. et al.1982. Transmissible genetic factors in Bac. thuringiensis:reasons for difference in some biochemical properties in wild type strains of Bac. thuringiensis var. gallerea. Genetika(USSR) 18,1069-68. Ambartsumian N.S. et al.1987 "Comparative characterisation of extrachromosomal DNA of Bac.thuringiensis serotype H-14

strains.Microbiologiya (USSR) 56,243-48.

Zakharyan's articles are referenced ("not in some books"- as you presented to Smartse), but , as you found in GOOGLE-books,referenced by world recognised scienists with outstanding contributions in science, including 6 what I have known before: In :"Fermentation process development of industrial organisms", 1989, ISBN 0-8247-7917-7 . Edited by Justin O. Neway. Marcel Dekker INC. N.Y.(Zakharyan R.A.etal.1979) In :"Advances in microbial control of insect pests", 2003, ISBN 0-306-47491-3. Edited by R. K. Upadhyay.Kluver Academy Plenum Publisher.N.Y. ( Zakharyan R.A.1976) In :Pathogens of invertebrates: application in biological control and ... - Page 159 Thomas Clement Cheng, Society for Invertebrate Pathology. Meeting - Science - 1984 - 278 pages,(Zakharyan R.A.et al. 1976,They later isolated three plasmids from B. thuringiensis var. caucasicus.Zakharyan et al. , 1979). In :Revista biología, Volumes 12-14 - Page 84 Universidad de La Habana. Facultad de Biología - Science,El primer trabajo que demuestra la presencia de plásmidos en Bacillus Thuringiensis data de 1976 (Zakharyan 1976) ya partir de ahí, ... In: Bioprocess engineering: the first generation - Page 290 Tarun K. Ghose - Science - 1989 - 389 pages.(Zakharyan R.A.et al.1976). In: The Molecular biology of the bacilli, Volume 2 David A. Dubnau - Science - 1985 - 259 pages(Zakharyan R.A.et al. 1976,1979) .

In 2003 Zakharyan's 1976 paper was referenced in book edited by R.K. Upadhyay(see above). In 006 Zakharyan"s 1976 paper was referenced in Review : Entomological Journal of East CHINA:XU Jian, LIU Qin, YIN Xiang-dong and ZHU Shu-de (2006). "A review of recent development of Bacillus thuringiensis ICP genetically engineered microbes". Entomological Journal of East China 15 (1): 53–58.. AIRcorn, Now read what are saying in scientific press scientist with the outstanding contribution in the BT, Dean D.H. , Clayton C. Beegle , Takhashi Yamamoto in their review articles about Zakharyan's 1976,1979 work and discovery (see above):exact the words and phrase that you have removed from text and articles from references:"Zakharyan R. A.(1976,1979) first reported the presence of plasmids in B.thuringiensis and suggested involvement of the plasmids in endospore/crystal formation,also described the presence of large plasmid in Cry+ variant of B.thuringiensis."; and also look XU Jian et al. article(2006)(Zakharyan R.A. 1976); and other authors.

Zakharyan's name has been presented among names of 26 scientists evalueted with landmark work in Bt. since 1901( I.S.Ishiwata's discovery). So,the information that you have and provided to Smartse" that information to you comes up very little and that paper(you presented just only one, Landen R et al.) does mention him (Zakharyan) also mentions others" and other statements are irrelevant, and the statement mentioned by you to Smartse that"His 1979 paper seems to be the most cited" and only "can be used as a reference" also irrelevant and I am sure because of lack of good information. The discovery of plasmids in Bt and experimental evidence suggested involvement of the plasmids in endospore /crystal formation by Zakharyan R.A. was followed by intensive research to characterise plasmids and determine their role in Bt-endotoxin production, followed by growing Bt -recombinant technology and industry.In this way remarkable and outstanding work was done by J. Gonsales ,H.E.Schnepf and H.R.Whiteley. And one more,articles in Wikipedia to be more encyclopedic should include names and even photo of #1 contributors in the field.I am looking forward to see new version of your article asap.Erkad,talk. Hi AIRcorn, In article Bt I did small change 1979 to 1976.Thank's for prompt answer.Erkad,talk.

I have copied your response from Smartse's talk page and replied to it. I am also in the process of updating the Bacillus Thuringiensis article. Cheers AIRcorn (talk) 07:17, 24 February 2010 (UTC)

Hi AIRcorn, In article Bt I did small change 1979 to 1976.Thank's for prompt answer.Erkad,talk.

Hi AIRcorn,to be consistent in Bt. "Discovery and Study" please consider, that the enormous progress in Bt -recombinant technology followed by today's Bt.-industry was a result of a discovery of plasmids and a large one identified in Cry+ strain with evidence suggesting that crysal protein is specified by plasmid DNA (Zakharyan 1976,1979) , discovery that a large plasmid has been associated with crystal protein production( Gonzales JM et al. 1981. Correlation between specific plasmids and endotoxin production in Bt. Plasmid 5,351-365.), and recombinant technology successfuly was applied for cloning crystal toxin gene (Schnepf H.E. and Whiteley H.R. 1981. Cloning and expression of the Bt. crystal protein gene in E.coli. Proc.Natl.Acad. Sci. USA 78,2893-2897.). Facts speak for themselves.Cheers Erkad (talk).tures|unsigned]] comment added by Erkad (talk •