User:Rosieredfield/draft Competence article

This sub-page of my User page is where I will create a revision of the article on Natural Competence (= 'Competence (biology)').

In microbiology, genetics, cell biology and molecular biology, competence is the ability of a cell to take up extracellular DNA from its environment. The term competence is used for two processes: 1. natural competence, the genetically specified expression of DNA uptake machinery by certain bacteria which enables them to actively take up DNA fragments both under natural conditions and in the laboratory; and 2. artificial competence, treatment of cells in laboratory cultures to make them transiently permeable to DNA provided in the external medium. This article primarily deals with natural competence in bacteria. Information about artificial competence is provided in a separate article.

In this image, a DNA fragment from the environment of a naturally competent Gram-negative bacterial cell is transported across the cell's two membranes into its cytoplasm. The steps of uptake are 1. Initiation of uptake at the cell surface; 2. Uptake of the DNA across the outer membrane (o.m.); 3. Translocation of a single DNA strand across the cell's inner membrane (i.m.); 4. Recombination of the incoming strand with homologous sequences in the cell's chromosome.

History

Natural competence was discovered by Frederick Griffith in 1928, when he showed that a preparation of killed cells of a pathogenic bacterium contained something that could transform related non-pathogenic cells into the pathogenic type. In 1944 Oswald Avery, Colin MacLeod, and Maclyn McCarty demonstrated that this 'transforming factor' was pure DNA. This was the first compelling evidence that DNA carries the genetic information of the cell.

Since then, natural competence has been studied in a number of different bacteria, particularly Bacillus subtilis, Streptococcus pneumoniae (Griffith's "pneumococcus"), Neisseria gonorrhoeae and Haemophilus influenzae. Areas of active research include the mechanisms of DNA transport, the regulation of competence in different bacteria, and the evolutionary function of competence.

Sources of DNA

In the natural world DNA usually becomes available when other cells die and their contents spill into the environment (lysis). Because DNA is chemically very stable it accumulates to high concentrations anywhere where dead biomaterials accumulate (for example lake and ocean sediments). DNA is also abundant in the ocean and on wet surfaces where bacteria grow in biofilms. In laboratory studies DNA is provided by the researcher, either as chromosomal DNA from related cells with specific genetic properties or as genetically engineered fragments or plasmids.

Mechanisms of DNA uptake

Mechanisms of DNA uptake are very similar across all naturally competent bacteria, with only a few exceptions. During uptake, DNA is transported across the cell membrane(s), and the cell wall if one is present. In Gram-negative bacteria, the first steps are binding of DNA to the cell-surface uptake machinery, initiation, and progressive uptake of the long filament of double-stranded DNA across the bacterial outer membrane into the periplasmic space between the outer and inner membranes. The force that pulls the DNA is thought to be generated by motor proteins of the type IV pilus family. Gram-positive bacteria have a thick cell wall instead of an outer membrane, and their related motor proteins pull DNA through the cell wall to their single membrane, where it may be cut by a specific nuclease. In both Gram-negative and Gram-positive bacteria, a single strand of DNA is then translocated across the inner membrane into the cytoplasm and the other strand is degraded to its nucleotide subunits.

Most competent bacteria are thought to take up all DNA molecules with roughly equal efficiencies, but bacteria in the families Neisseriaceae and Pasteurellaceae preferentially take up DNA fragments containing short DNA sequences, termed DNA uptake sequence (DUS and USS respectively), that are very frequent in their own genomes. Neisserial genomes contain thousands of copies of the preferred sequence GCCGTCTGAA, and Pasteurellacean genomes contain either AAGTGCGGT or ACAAGCGGT.

Once the DNA is inside the cell it may be degraded to nucleotides, which are reused for DNA replication and other metabolic functions. Alternatively it may be recombined into the cell’s genome by its DNA repair enzymes, if the two DNAs have sufficiently similar sequences. If this recombination changes the cell’s genotype the cell is said to have been transformed (see Transformation (genetics)).

Regulation of competence

In laboratory cultures natural competence is usually tightly regulated and often triggered by nutritional shortages or adverse conditions. However the specific inducing signals and regulatory machinery are much more variable than the uptake machinery, and little is known about the regulation of competence in the natural environments of these bacteria. Although the regulatory signals that control competence development might be expected to shed light on its evolutionary function (see below), their great variety and complexity make this such interpretation difficult.

Transcription factors have been discovered which regulate competence; an example is sxy (also known as tfoX) which has been found to be regulated in turn by a 5' non-coding RNA element. In bacteria capable of forming spores, conditions inducing sporulation often overlap with those inducing competence. Thus cultures or colonies containing sporulating cells often also contain competent cells. Recent research by Süel et al. has identified an excitable core module of genes which can explain entry into and exit from competence when cellular noise is taken into account.

Evolutionary functions and consequences of competence

Most proposals made for the primary evolutionary function of natural competence as a part of natural bacterial transformation fall into three categories: (1) the selective advantage of genetic diversity; (2) DNA uptake as a source of nucleotides (DNA as “food”); and (3) the selective advantage of a new strand of DNA to promote homologous recombinational repair of damaged DNA (DNA repair). A secondary suggestion has also been made, noting the occasional advantage of lateral gene transfer.

Hypothesis of genetic diversity

Arguments to support genetic diversity as the primary evolutionary function of sex (including bacterial transformation) are given by Barton and Charleworth . and by Otto and Gerstein. However, the theoretical difficulties associated with the evolution of sex suggest that sex for genetic diversity is problematic. Specifically with respect to bacterial transformation, competence requires the high cost of a global protein synthesis switch, with, for example, more than 16 genes that are switched on only during competence of Strepococcus pneumoniae. However, since bacteria tend to grow in clones, the DNA available for transformation would generally have the same genotype as that of the recipient cells. Thus, there is always a high cost in protein expression without, in general, an increase in diversity. Other differences between competence and sex have been considered in models of the evolution of genes causing competence; these models found that competence's postulated recombinational benefits were even more elusive than those of sex.

Hypothesis of DNA as food

The second hypothesis, DNA as food, relies on the fact that cells that take up DNA inevitably acquire the nucleotides the DNA consists of, and, because nucleotides are needed for DNA and RNA synthesis and are expensive to synthesize, these may make a significant contribution to the cell's energy budget. Some naturally competent bacteria also secrete nucleases into their surroundings, and all bacteria can take up the free nucleotides these nucleases generate from environmental DNA. The energetics of DNA uptake are not understood in any system, so it is difficult to compare the efficiency of nuclease secretion to that of DNA uptake and internal degradation. In principle the cost of nuclease production and the uncertainty of nucleotide recovery must be balanced against the energy needed to synthesize the uptake machinery and to pull DNA in. Other important factors are the likelihoods that nucleases and competent cells will encounter DNA molecules, the relative inefficiencies of nucleotide uptake from the environment and from the periplasm (where one strand is degraded by competent cells), and the advantage of producing ready-to-use nucleotide monophosphates from the other strand in the cytoplasm. Another complicating factor is the self-bias of the DNA uptake systems of species in the family Pasteurellaceae and the genus Neisseria, which could reflect either selection for recombination or for mechanistically efficient uptake.

Hypothesis of repair of DNA damage

In bacteria, the problem of DNA damage is most pronounced during periods of stress, particularly oxidative stress, that occur during crowding or starvation conditions. Under such conditions there is often only a single chromosome present. The finding that some bacteria induce competence under such stress conditions, supports the third hypothesis, that transformation exists to permit DNA repair. In experimental tests, bacterial cells exposed to agents damaging their DNA, and then undergoing transformation, survived better than cells exposed to DNA damage that did not undergo transformation (Hoelzer and Michod, 1991). In addition, competence to undergo transformation is often inducible by known DNA damaging agents (reviewed by Michod et al., 2008 and Bernstein et al., 2012). Thus, a strong short-term selective advantage for natural competence and transformation would be its ability to promote homologous recombinational DNA repair under conditions of stress.

A counter argument was made based on the 1993 report of Redfield who found that single-stranded and double-stranded damage to chromosomal DNA did not induce or enhance competence or transformation in B. subtilis or H. influenzae, suggesting that selection for repair has played little or no role in the evolution of competence in these species

However more recent evidence indicates that competence for transformation is, indeed, specifically induced by DNA damaging conditions. For instance, Claverys et al. in 2006 showed that the DNA damaging agents mitomycin C (a DNA cross-linking agent) and fluoroquinolone (a topoisomerase inhibitor that causes double-strand breaks) induce transformation in Streptococcus pneumoniae. In addition, Engelmoer and Rozen in 2011 demonstrated that in S. pneumoniae transformation protects against the bactericidal effect of mitomycin C. Induction of competence further protected against the antibiotics kanomycin and streptomycin. Although these aminoglycoside antibiotics were previously regarded as non-DNA damaging, recent studies in 2012 of Foti et al. showed that a substantial portion of their bactericidal activity results from release of the hydroxyl radical and induction of DNA damages, including double-strand breaks.

Dorer et al., in 2010, showed that ciprofloxacin, which interacts with DNA gyrase and causes production of double-strand breaks, induces expression of competence genes in Helicobacter pylori, leading to increased transformation. In 2011 studies of Legionella pneumophila, Charpentier et al. tested 64 toxic molecules to determine which ones induce competence. Only six of these molecules, all DNA damaging agents, strongly induced competence. These molecules were norfloxacin, ofloxacin and nalidixic acid (inhibitors of DNA gyrase that produce double strand breaks), mitomycin C (which produces inter-strand cross-links), bicyclomycin (causes single- and double-strand breaks), and hydroxyurea (causes oxidation of DNA bases). Charpentier et al. also showed that UV irradiation induces competence in L. pneumophila and further suggested that competence for transformation evolved as a response to DNA damage.

Lateral gene transfer

A long-term advantage may occasionally be conferred by infrequent instances of lateral gene transfer (which might occur through non-homologous recombination after competence is induced), that could provide for antibiotic resistance or other advantages.

Regardless of the nature of selection for competence, the composite nature of bacterial genomes provides abundant evidence that the lateral gene transfer caused by competence contributes to the genetic diversity that makes evolution possible.

See also

• Transformation (genetics)

Category:Biology terminology