Welcome to my sandbox.
Here is where I practice making things bold and adding hyperlinks.
Biosynthesis edit
Ta-siRNAs arise from non coding transcripts through miRNA guided cleavage and conversion to double stranded RNA by the RNA-dependent RNA polymerase RDR6 and suppressor of gene silencing 3 (SGS3).[1] The resulting dsRNA is further processed by dicer-like enzyme 4 (DCL4) to produce a phased array of 21-nt siRNAs.[2][3]
Model for ta-siRNA biosynthesis initiation from Allen paper. Practice adding image here.
Text for refs < ref name=xyz>YOUR SOURCE</ref> ref tags
Presence of ta-siRNAs edit
The presence of ta-siRNAs was originally detected in the flowering plant Arabidopsis [3]. Evidence of ta-siRNAs has also been found in the moss Physcomitrella patens [1], maize [4], and a strain of Oryza sativa rice. [5][4]
References edit
- ^ a b Talmor-Neiman M, Stav R, Klipcan L, Buxdorf K, Baulcombe DC, Arazi T (November 2006). "Identification of trans-acting siRNAs in moss and an RNA-dependent RNA polymerase required for their biogenesis". Plant J. 48 (4): 511–21. doi:10.1111/j.1365-313X.2006.02895.x. PMID 17076803.
{{cite journal}}: CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link) - ^ Xie Z, Qi X (November 2008). "Diverse small RNA-directed silencing pathways in plants". Biochim. Biophys. Acta. 1779 (11): 720–4. doi:10.1016/j.bbagrm.2008.02.009. PMID 18358247.
{{cite journal}}: CS1 maint: date and year (link) - ^ a b Allen E, Xie Z, Gustafson AM, Carrington JC (April 2005). "microRNA-directed phasing during trans-acting siRNA biogenesis in plants". Cell. 121 (2): 207–21. doi:10.1016/j.cell.2005.04.004. PMID 15851028.
{{cite journal}}: CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link) - ^ a b Williams L, Carles CC, Osmont KS, Fletcher JC (July 2005). "A database analysis method identifies an endogenous trans-acting short-interfering RNA that targets the Arabidopsis ARF2, ARF3, and ARF4 genes". Proc. Natl. Acad. Sci. U.S.A. 102 (27): 9703–8. doi:10.1073/pnas.0504029102. PMC 1172271. PMID 15980147.
{{cite journal}}: CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link) - ^ Heisel SE, Zhang Y, Allen E; et al. (2008). "Characterization of unique small RNA populations from rice grain". PLOS ONE. 3 (8): e2871. doi:10.1371/journal.pone.0002871. PMC 2518513. PMID 18716673.
{{cite journal}}: Explicit use of et al. in:|author=(help)CS1 maint: multiple names: authors list (link)