The agarose gel is stained with ethidium bromide.[1] Ethidium bromide’s unique ring structure enables it to stack into the DNA strand thus working as an intercalating agent.[2] This chemical stain helps us analyze the PCR products within the gel by enhancing their emission properties[3].
- ^ "Polymerase Chain Reaction". Journal of Investigative Dermatology. 133 (3): 1–4. 2013-03-01. doi:10.1038/jid.2013.1. ISSN 0022-202X.
- ^ Lin, Po-Chen; Chang, I-Jy (2015-09-01). "Photophysical Properties of Ethidium Bromide in Low Concentration of Sodium Dodecyl Sulfate". Journal of the Chinese Chemical Society. 62 (9): 780–785. doi:10.1002/jccs.201500165. ISSN 2192-6549.
- ^ Lin, Po-Chen; Chang, I-Jy (2015-09-01). "Photophysical Properties of Ethidium Bromide in Low Concentration of Sodium Dodecyl Sulfate". Journal of the Chinese Chemical Society. 62 (9): 780–785. doi:10.1002/jccs.201500165. ISSN 2192-6549.