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Edits edit
Genomics edit
Early Sequencing efforts edit
"Rosalind Franklin's confirmation of the helical structure of DNA was one of the earliest sequencing efforts. In 1951, Rosalind and fellow student Raymond Gosling, were able to capture two high-resolution photos of crystallized DNA fibers. "Franklin used two different fibers of DNA, one more highly hydrated than the other. From this she deduced the basic dimensions of DNA strands, and that the phosphates were on the outside of what was probably a helical structure."" [1] Exactly 16 months later, J. D. Watson and F. Crick published their description of DNA, which was based on Franklin's x‐ray photos. [2]
Recombinant DNA edit
History edit
"In 1971, Douglas Berg isolated the first plasmid bacterial cloning vector, λdvgal 120 and Robert Pollack voiced his concern about the potential biohazards of cloning. A year later in 1972, David Jackson, Peter Lobban, and A. D. Kaiser developed a method, which allowed the joining of DNA's in vitro and Jackson and colleagues created the first chimeric DNA in vitro. [3] "In 1973, for the first time ever S.Cohen and H. Boyer developed a recombinant plasmid, which after using as vector replicated well within a bacterial host." [4]
Suggested Edits edit
Genomics edit
My Suggested Edits:
Early sequencing efforts
Early sequencing efforts edit
I believe that the section "early sequencing efforts" lacks information and is quite vague with the different efforts and publications that took place. I think that each effort/publication should have had a short summary explaining research that was found and what was accomplished. I think that the first sentence should say: Rosalind Franklin's confirmation of the helical structure of DNA was one of the earliest sequencing efforts. In 1951, Rosalind and an fellow student named Raymond Gosling, were able to capture two high-resolution photos of crystallized DNA fibers. "Franklin used two different fibers of DNA, one more highly hydrated than the other. From this she deduced the basic dimensions of DNA strands, and that the phosphates were on the outside of what was probably a helical structure." [5] AlejandroArias (talk) 07:00, 21 April 2017 (UTC)
Recombinant DNA edit
My Suggested Edits:
History
Controversy
History edit
I agree with both Jackierodz518 and Abbystewart that more information needs to be added to the History section. I believe that having more background information/history, will give readers a better understanding of the material. In addition, I think that information should be added regarding all of the money hungry corporations that wanted to capitalize on Stanley N. Cohen and Herbert W. Boyle's work, which led to the birth of the Biotech Industry. AlejandroArias (talk) 18:58, 20 April 2017 (UTC)
After the first sentence I would add: "In 1971, Douglas Berg isolated the first plasmid bacterial cloning vector, λdvgal 120 and Robert Pollack voiced his concern about the potential biohazards of cloning. I would continue with another sentence and say: A year later in 1972, David Jackson, Peter Lobban, and A. D. Kaiser developed a method, which allowed the joining of DNA's in vitro and Jackson and colleagues created the first chimeric DNA in vitro. [6]
Controversy edit
The "Controversy" section is missing crucial information regarding cloning. I believe that there should be a point emphasizing the public fear of cloning and the debates/conferences that took place during the 1970's. In addition, there should be information regarding the impact these debates had on society. These recombinant DNA debates impacted many different sectors of society. These sectors included, the courts, Congress, the executive branch, interagency committees, foreign governments, the National Academy of Sciences, professional societies, the new biotechnology industry, university scientists, local governments and most importantly the media. AlejandroArias (talk) 21:50, 20 April 2017 (UTC)
- ^ "Rosalind Franklin :: DNA from the Beginning". www.dnaftb.org. Retrieved 2017-04-21.
- ^ "How Rosalind Franklin Discovered the Helical Structure of DNA: Experiments in Diffraction". The Physics Teacher. 49 (3): 140–143. 2011-02-17. doi:10.1119/1.3555496. ISSN 0031-921X.
- ^ Lenzi, Rebecca N.; Altevogt, Bruce M.; Gostin, Lawrence O.; Committee, Committee on the Independent Review and Assessment of the Activities of the NIH Recombinant DNA Advisory; Policy, Board on Health Sciences; Medicine, Institute of (2014-03-27). Historical and Policy Timelines for Recombinant DNA Technology. National Academies Press (US).
{{cite book}}:|last5=has generic name (help) - ^ "Recombinant DNA Technology: Definition and History | Genetics". Biology Discussion. 2016-11-28. Retrieved 2017-04-21.
- ^ "Rosalind Franklin :: DNA from the Beginning". www.dnaftb.org. Retrieved 2017-04-21.
- ^ Lenzi, Rebecca N.; Altevogt, Bruce M.; Gostin, Lawrence O.; Committee, Committee on the Independent Review and Assessment of the Activities of the NIH Recombinant DNA Advisory; Policy, Board on Health Sciences; Medicine, Institute of (2014-03-27). Historical and Policy Timelines for Recombinant DNA Technology. National Academies Press (US).
{{cite book}}:|last5=has generic name (help)