User:Aargudo/sandbox

Biochemistry Lab:

possible topic of for wikipedia: Bradford Reagent

Class knowledge-

 The Bradford Method is an analytical procedure that determines the concentration of protein within a sample.

Dye used: “Coomassie Brilliant Blue G-250” which forms a non-covalent complex with proteins.

How does the dye interact: Through electrostatic and Van der Waals interactions, the dye causes a disruption within the protein.

1.Electrostatic interactions occur between ionizable groups, like amino and carboxyl groups of the proteins to the electrons from the Dye.

2.non-covalent interactions allow the dye to be able to interact with the protein. 

Absorption changes-

Once the dye and protein interact, the absorption maximum changes from 495nm to 595nm. The absorptivity change is indicative of a successful interaction.

Pros and cons of the bradford method-

1.Bradford method has many advantages and one of them is that it can be used on certain proteins that have been reduced by reducing agents, which separates it from other methods like Lowry protein assay.

2.Drawbacks to Bradford include its incompatibility with detergents, which at times can be used to solubilize membrane proteins.

website resources:

1.https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/b6916bul.pdf (can't use because not scientific journal.)

2.Scientific Journal: https://ac.els-cdn.com/S000326970800763X/1-s2.0-S000326970800763X-main.pdf?_tid=2160a1e6-de92-11e7-b3c4-00000aacb35e&acdnat=1513010666_fb9fd5ae9e933aa96f2e6c5bda59ba2a

(from hunter database so I have to cite properly)

discusses:

-Compatiable with reducing agents

Where I want to add/discuss? Section of advantages, comparing this method to other analytical procedure used to measure the concentration of proteins within a solution.

Topic: Ability to interact with reducing agent unlike other protein analytical procedures ( Lowry and BCA)

Regarding reducing agents:

Reducing agents like Dithiothreitol (DTT), B-Mercaptoethanol are routinely used in protein samples. You can remove these interfering substance through precipitation (by adding acetone or TCA)

Citations for websites: Wikipedia:Citing sources

URL of the web page – that is the URL of the web page where the referenced content can be found, not, e.g., the main page of a website when the content is on a subpage of that website (see Wikipedia:Shallow references),name of the author(s),title of the article within quotation marks, translated title of the article in square brackets after the title if not in English,title or domain name of the website,publisher, if known date of publication,page number(s) (if applicable), the date you retrieved (or accessed) the web page (required if the publication date is unknown.

Example:

section I want to add to: (underline means changed)

Protein samples usually contains salts, solvents, buffers, preservatives, reducing and metal chelating agents. These molecules are frequently used for solubilizing and stabilizing proteins. Other protein assay like BCA and Lowry are ineffective because molecules like reducing agents interfere with the assay. Using Bradford can be advantageous against these molecules because they are compatible to each other and will not interfere.

New sections: From journal****

"The assay has a moderate protein-to-protein variation and is compatible with most detergents, reducing agents, and other commonly used reagents" 

"The BCA assay is related to the Lowry assay...the disadvantage of the assay is the fact that reducing agents interfere with the assay."

"The most useful features to consider when choosing a protein assay are sensitivity, compatibility with common substances in samples"

"Because detergents and reducing agents are frequently used for solubilizing and stabilizing proteins, this assay has a significant advantage"

youtube videos: wikipedia editing basics

For our group:

1. Sentence/phrase you wish to contribute. (separate from the group).
2. full group paragraph
3. copying and editing source?
4. copy whole thing (ctrl-c), then go to the article, choose the specific section (and choose edit source) use wikicode (copy and paste what you want to add), then save it.
5. things to notice? citation becomes correctly ordered, and to verify edit? check history and see recent edits.

Resources for abstract of Puerto Rican History:

I found this article here:

News paper about Ponce Massacre

Journal about Ponce Massacre (starts from Un Domingo de Lilianne [Lilianne's Sunday])

Potential editing:

On March 21, 1937, a peaceful march was organized by the Nationalist Party to commemorate the ending of slavery in Puerto Rico in 1873 by the governing Spanish National Assembly. The police, under the orders of General Blanton Winship, the US-appointed colonial Governor of Puerto Rico, opened fire at the peaceful Puerto Rican Nationalist Party parade, bringing about what came to be known as the "Ponce massacre": 19 people (including two policemen) were killed and over 100 were wounded.^[55]

Fixing in the Article:

• Confirmation of leaders.
• Adding new leaders, as per needed.
• Confirmation of stats; making stats more precise
• Confirmation of dates

Who is governing the Spanish National Assembly in 1873 + link/footnote

Cristino Martos? There is no concrete evidence that he was in charge of the Spanish Nationalist Assembly or influenced the end of slavery in Puerto Rico.

Who is governing the Nationalist Party? + link/footnote

Pedro Alibizu Campos. 2 outside sources and wikipedia link

Pedro Albizu Campos Pedro Albizu Campos Pedro Albizu Campos

Dates and fact check. (more specific, like month and year.)

Group Contribution.

Both Sal and Abdullah fact checked the statistics regarding the death toll of the Ponce Massacre and accuracy of the wounded and individuals involved in the massacre. Sal also assisted in adding citations and resources into the article when we corrected it.

Sonia searched information regarding the Spanish National Assembly but found no concrete source regarding the leader of the Spanish National Assembly or who ended slavery in Puerto Rico. She also researched about the dates these significant events occurred and focused on editing the article, checked for grammar and punctuation.

Divide by organizing and confirming dates, confirming leaders and fact check.

Final edit:

On March 21, 1937, a peaceful march was organized by the Nationalist Party, under Pedro Alibizu Campos, to commemorate the ending of slavery in Puerto Rico in 1873 by the governing Spanish National Assembly. The police, under the orders of General Blanton Winship, the US-appointed colonial Governor of Puerto Rico, opened fire at the peaceful Puerto Rican Nationalist Party parade, which is now known as the "Ponce massacre": 20 unarmed people (including two policemen) were killed, with wounded persons ranging between 100-200. 

My assignment: adding and confirming leaders.