Transmembrane protein 254

Transmembrane protein 254 is a transmembrane protein that is encoded by the TMEM254 gene, it is predicted to have many orthologs across eukaryotes.

Transmembrane protein 254
Identifiers
SymbolTMEM254
Alt. symbolsC10orf57
NCBI gene80195
HGNC25804
RefSeqNM_001270367
UniProtQ8TBM7
Other data
LocusChr. 10 q22.3
Search for
StructuresSwiss-model
DomainsInterPro

Gene

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TMEM254 is located on the long arm of chromosome 10, open reading frame 57 on the positive strand. It is found at position 10q22.3. It has seven known transcript variants.[1]

 

This image shows the cytogenetic band of human chromosome 10 and the various gene locations on it. TMEM254 can be seen at the thin red band with the box around, placed at q22.3.[2]

mRNA

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TMEM254 consists of ten total exons and is 13,912 bp in length.[3]

Isoforms TMEM254 has 3 isoforms produced by alternative splicing.[4]

Isoform Length Mass (Da)
1 123 14,243
2 84 9,692
3 103 12,068

Protein

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Predicted structure of TMEM254 derived from I-TASSER[5]

TMEM254 contains:[6][7]

The secondary structure of TMEM254 is predicted to consist of alternating pairs of alpha helices and beta sheets.

Expression

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Human Protein Atlas shows TMEM254 expression.[9]

According to a human GEO profile and human and mouse EST profiles, TMEM254 appears to be expressed at a relatively high level in most tissues.[10][11]

TMEM254 is highly expressed in reproductive structures, and a wide range of cells. These include, but are not limited to, thyroid gland, pancreas, kidney, skin, breasts, and testes.

Homology

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There are no known paralogs for transmembrane protein 254, however it does have various orthologs within orthologs within eukaryotes.[12] The following table presents some of the orthologs found using searches in BLAST.[13] A comparison of a wide variety of known TMEM254 orthologs meant to display the diversity of species for which orthologs are found is listed in the table below:[14]

Organism Common Name Divergence from

Humans (MYA)

Accession Number Sequence Length Sequence Identity Sequence Similarity Notes
Homo sapiens Humans N/A NP_001257296 N/A 100% 100% Mammalia
Pan troglodytes Chimpanzee 6.40 XP_003312695 6.4 99.3% 99.3% Mammalia
Rhinopithecus bieti Black snub-nosed monkey 28.1 XP_011886260 28.1 95.2% 97.3% Mammalia
Chrysemys pictabellii Painted Turtle 320 XP_008168753 320 46.3% 57.8% Reptilia
Esox lucius Northern Pike 320 NP_001290628 432 45% 60.4% Actinopterygii
Alligator mississippiensis American Alligator 320 KYO27663 320 43.3% 56.7% Reptilia
Nanorana parkeri High Himalaya frog 353 XP_018428933 353 42.3% 57.7% Amphibia
Phaethon lepturus White-tailed Tropicbird 320 XP_010286446 320 38.8% 59.3% Aves
Hippocampus comes Tiger Tail Seahorse 432 XP_019737413 432 38.1% 56.5% Actinopterygii
Callorhinchus milii Elephant Shark 465 XP_007897415 465 37.9% 58.2% Chondrichthyes
Patagioenas fasciata monilis Band-tailed Pigeon 320 OPJ84646 320 36.7% 59.9% Aves
Saccaglossus kowalevskii Acorn Worm 627 XP_006821285 627 31.8% 51% Enteropneusta

Predicted Post-Translational Modification

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Conceptual Translation

Using various tools at ExPASy[15] the following are possible post-translational modifications for TMEM254.

  • 4 possible PKC phosphorylation sites
  • 3 possible PKA phosphorylation sites

References

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  1. ^ "TMEM254 transmembrane protein 254 [Homo sapiens (human)] - Gene - NCBI".
  2. ^ "TMEM254 Gene - GeneCards | TM254 Protein | TM254 Antibody".
  3. ^ "TMEM254 - Exons".
  4. ^ "TMEM254 - Transmembrane protein 254 - Homo sapiens (Human) - TMEM254 gene & protein".
  5. ^ "I-TASSER server for protein structure and function prediction".
  6. ^ "Transmembrane protein 254 isoform 2 [Homo sapiens] - Protein - NCBI".
  7. ^ "PSORT II Prediction".
  8. ^ "TMEM254 (human)".
  9. ^ "Tissue expression of TMEM254 - Summary - the Human Protein Atlas".
  10. ^ EST Profile Viewer- Human
  11. ^ EST Profile Viewer- Mouse
  12. ^ "TMEM254 Gene - GeneCards | TM254 Protein | TM254 Antibody".
  13. ^ "BLAST: Basic Local Alignment Search Tool".
  14. ^ "National Center for Biotechnology Information".
  15. ^ ExPASy Proteomics Server