A thiocyanate hydrolase (EC 3.5.5.8) is an enzyme belonging to the family of hydrolases. The systematic name of this enzyme class is thiocyanate aminohydrolase. This enzyme catalyzes the chemical reaction:

thiocyanate hydrolase
Identifiers
EC no.3.5.5.8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins
SCN + 2 H2O + H+ ⇌ SCO + NH3

The mechanism is proposed to involve a metal thiocyanate complex.

Structural studies

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As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 2DD4, 2DD5, 2DXB, and 2DXC.

A second thiocyanate hydrolase with copper at its active site catalyzes its conversion to cyanate:[1]

SCN + H2O → OCN + H2S

References

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  1. ^ Tikhonova, Tamara V.; Sorokin, Dimitry Y.; Hagen, Wilfred R.; Khrenova, Maria G.; Muyzer, Gerard; Rakitina, Tatiana V.; Shabalin, Ivan G.; Trofimov, Anton A.; Tsallagov, Stanislav I.; Popov, Vladimir O. (2020). "Trinuclear Copper Biocatalytic Center Forms an Active Site of Thiocyanate Dehydrogenase". Proceedings of the National Academy of Sciences. 117 (10): 5280–5290. Bibcode:2020PNAS..117.5280T. doi:10.1073/pnas.1922133117. PMC 7071890. PMID 32094184.