Testosterone 17beta-dehydrogenase

In enzymology, a testosterone 17beta-dehydrogenase is an enzyme that catalyzes the chemical reaction between testosterone and androst-4-ene-3,17-dione. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.

testosterone 17-beta-dehydrogenase (NAD+)
Identifiers
EC no.1.1.1.63
CAS no.9028-62-0
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BRENDABRENDA entry
ExPASyNiceZyme view
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MetaCycmetabolic pathway
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testosterone 17-beta-dehydrogenase (NADP+)
Identifiers
EC no.1.1.1.64
CAS no.9028-63-1
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

Names

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The systematic name of this enzyme class is 17beta-hydroxysteroid:NAD+ 17-oxidoreductase. Other names in common use include 17-ketoreductase and 17beta-HSD. This enzyme participates in androgen and estrogen metabolism.

Variants

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There are two variants of the enzyme, one that uses NAD+ as a substrate, and one that uses NADP+ as acceptor.

NAD+

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This variant of testosterone 17beta-dehydrogenase (EC 1.1.1.239) catalyzes the reaction

testosterone + NAD+   androst-4-ene-3,17-dione + NADH + H+

Thus, the two substrates of this enzyme are testosterone and NAD+, whereas its 3 products are androst-4-ene-3,17-dione, NADH, and H+.

NADP+

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This variant of testosterone 17beta-dehydrogenase (EC 1.1.1.64) catalyzes the reaction

testosterone + NADP+   androst-4-ene-3,17-dione + NADPH + H+

Thus, the two substrates of this enzyme are testosterone and NADP+, whereas its 3 products are androst-4-ene-3,17-dione, NADPH, and H+.

See also

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References

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  • Endahl GL, Kochakian CD, Hamm D (1960). "Separation of a Triphosphopyridine Nucleotide-specific from a Diphosphopyridine Nucleotide-specific 17β-Hydroxy-(testosterone)dehydrogenase of Guinea Pig Liver". J. Biol. Chem. 235 (10): 2792–6. doi:10.1016/S0021-9258(18)64542-9. PMID 13696735.
  • Sweat ML, Samuels LT, Lumry R (1950). "Preparation and characterization of the enzyme which converts testosterone to androstenedione". J. Biol. Chem. 185 (1): 75–84. doi:10.1016/S0021-9258(18)56395-X. PMID 15436478.
  • Villee CA; Spencer JM (1960). "Some Properties of the Pyridine Nucleotide-specific 17β-Hydroxy Steroid Dehydrogenases of Guinea Pig Liver". J. Biol. Chem. 235 (12): 3615–3619. doi:10.1016/S0021-9258(18)64518-1. PMID 13781425.
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