Talk:Twister ribozyme

Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): JRHall. Peer reviewers: Jdogzz.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 04:30, 18 January 2022 (UTC)

Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): McFarlR.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 04:30, 18 January 2022 (UTC)

Suggestions for Improvement

The only section I feel could use more elaboration is the "Discovery" section, where you might describe who did the study, and more details about the work that led to their discovery. Other than that, I think in general you could include more internal Wikipedia references for terminology as you use it, and then maybe consider reading the YouTube template page with regard to your "See Also" section.-Jdogzz (talk) 21:20, 24 October 2017 (UTC)

The discovery section now includes a brief description of the bioinformatic approach and a link to the citation of their methodology. Additionally, I have just looked over the page and linked several Wikipedia references to terminology and topics. JRHall (talk) 19:39, 30 October 2017 (UTC)

You should also include a brief caption for the figure you've added to the article.-Jdogzz (talk) 17:00, 31 October 2017 (UTC)

I agree with Jdogzz's comments. On another note, is the twister sister ribozyme exactly the same thing as this or is it different? It uses the same reference from Roth A. M bartolo (talk) 02:05, 25 October 2017 (UTC)

No, the twister sister ribozyme is not the same as the twister ribozyme, but they do have similar functionality and "vague" structure similarities. The two ribozymes do not share a reference; while both share a bioinformatics based discovery the citations are not from the the same reference, but they do share some contributing authors. JRHall (talk) 19:10, 30 October 2017 (UTC)

I suggest adding some specifics about the reaction, i.e., cis- vs. trans-acting. Can you also clarify, when you say the catalytic activity does not depend on Mg2+ or other cations but secondary structure does, I assume you mean that there are divalent cations observed to stabilize the structure but they do not play a direct role in catalysis? That is a bit different from how it is currently phrased, that the ribozyme does not depend on Mg2+ for catalytic activity (since the activity presumably does depend on having an intact structure). Also, if there is a known crystal structure it would be nice to have that displayed here. Some details on the initial bioinformatic identification would also be helpful, as others mention above. Ireneachen (talk) 19:54, 26 October 2017 (UTC)

I have made several additions to the content and have clarified language in a few sentences throughout the article. Further, you will now find an image of the crystal structure resolved at 2.3 A that I have prepared in Pymol. JRHall (talk) 03:18, 1 November 2017 (UTC)