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Untitled
editIn my editing of this page I have jumped around some between the past and present tense. I've done my best to correct this, but I'm pretty sure I didn't catch all my mistakes. Ian Glenn 22:47, 3 April 2007 (UTC)
I basically rewrote the article. It was very poorly written by myself when I expanded on the stub in december 2006. The content is correct now. Here are some issues I thought necessary to raise based on some of the content that found its way into the article in the past.
Restriction Mapping as opposed to diagnostic restriction digests A diagnostic digest to determine insert orientation is a commonly used application of restriction mapping. It is not the definition of restriction mapping. In the article this kind of digest is used as an example.
The Difference between RFLP and Restriction mapping
They use Restriction fragment length polymorphisms in to differentiate individuals of the same species with 99.999999999999% identical genomes. For RFLP they just compare the digest of two samples to see whether they were from the same individual. This was the original way of doing forensic DNA matching for solving murders and stuff. For small bacterial genomes the mapping position of sites may not change as much from individual to individual. RFLP was used for screening for certain alleles too, and for some mapping on a larger scale. However bringing RFLP into this can be confusing, and can bring the article off topic. RFLP and restriction mapping are related techniques, but not the same thing.
There is an article on Restriction Enzymes already I deleted the part that tells people what restriction enzymes do, and that described RFLP incorrectly (it was too un-thourough to be considered correct).
Restriction mapping on the chromosome requires southern blotting to look for certain fragments in the smear the genomic digest produces. It is used for mapping the position of transposons that have inserted aswell.
Anyway, the point is, it's not practical to talk about every application you would use restriction enzymes for in this article. The article is just trying to get the principle of the method across.
Perhaps it would be good to put in a section with a list of applications.
Exhilaration157 22:00, 22 November 2008