Talk:MTORC2

Class Project

Hi mTOR editors, this is Bobby, a student in a Signal Transduction class at Saint Louis University. Each student is allowed to pick a topic that they found exciting as well as a topic they felt could expand the current wikipedia knowledge on. This article, along with mTOR and mTORC1 are going to be what I am going to be editing for the rest of the semester. If you have any help or input or advice, don't hesitate to leave a comment on my talk page. I am planning on, for the editing, reading upwards of twenty review articles (and perhaps a couple primary articles) about mTOR and its signaling within the cell and using that information to help (hopefully) add to the current knowledge about this protein (these proteins) already supplied on Wikipedia. This is just to help give a heads up that I am working on this.

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This article is part of an assignment from Saint Louis University in Spring 2013 (see the course page for more details).

Thanks,Flemingrjf (talk) 03:22, 18 February 2013 (UTC)

Comments from Gpruett2

Hey Bobby, here are my suggestions for your contribution to this article:

• Your citations are solid and support the information that you have added.
• My main suggestion for your additions would be to expound upon them. The main reason for this is that as they are now, it is difficult to see how they relate to Regulation.
• Finally, for the article in general, it needs a lot more information. It needs the same level of information that can be found for mTORC1. Gpruett2 (talk) 04:24, 27 March 2013 (UTC)

• You might find this reference useful: [1]Biolprof (talk) 02:53, 14 April 2013 (UTC)

Comments from Jnims

Great job so far! I've included my suggestions for improvement below as part of our Assignment 7. You'll notice that some are the same as for your main article (mTOR).

Lead section

• Add "itself" at the end of this phrase to underscore the presence of mTOR in the complex: "mTOR Complex 2 (mTORC1) is composed of mTOR."
  • Thus, the entire sentence would read, "mTOR Complex 2 (mTORC2) is composed of mTOR itself, rapamycin-insensitive companion of mTOR (RICTOR), GβL, and mammalian stress-activated protein kinase interacting protein 1 (mSIN1)."
• A PyMOL representation of the mTORC2 structure would be helpful.

Function section

• Remove extra words ("has been shown to") in this sentence, for the sake of brevity: "mTORC2 has been shown to function as an important regulator of the cytoskeleton through its stimulation of F-actin stress fibers, paxillin, RhoA, Rac1, Cdc42, and protein kinase C α (PKCα)."
  • Result: "mTORC2 functions as an important regulator of the cytoskeleton through its stimulation of F-actin stress fibers, paxillin, RhoA, Rac1, Cdc42, and protein kinase C α (PKCα)."
• Are you describing the function of PDK2 here? Please clarify. (There is also an unnecessary space after "serine residue S473" that I would have removed, but there is some wiki markup that I don't recognize and hesitate to edit.): "mTORC2 also appears to possess the activity of a previously elusive protein known as "PDK2". mTORC2 phosphorylates the serine/threonine protein kinase Akt/PKB at a serine residue S473 . Phosphorylation of the serine stimulates Akt phosphorylation at a threonine T308 residue by PDK1 and leads to full Akt activation;[20][21] curcumin inhibits both by preventing phosphorylation of the serine." Also, what do you mean by "inhibits both" in the last sentence?

Miscellaneous

• You may want to add a link to mTOR and mTORC1 as part of a "See also" section.

Jnims (talk) 01:18, 23 April 2013 (UTC)

Peer Review comments from MChapman5

• The "Regulation" section could have a lot more wiki-links.
• Since mTORC2 may play a role in cancer, should that be given its own section for "cancer role" or "disease state" like you did for mTORC1?
• If you could find a picture to add to the "function" section showing what/where it phosphorylates that could be beneficial. However, given the limited information in this article, I'm sure finding an image would be challenging. It could be a nice future contribution.
• Great start to this page! You've done wonderful with the entire mTOR section. MChapman5 (talk) 07:16, 30 April 2013 (UTC)

Peer Review: BreCaitlin

• How does mTORC2 regulate the cytoskeleton? What does it do?
• I know in regulation you say mTORC2 is suspected in cancer, is there anyway to make a separate section for diseases? I get if theres not enough information on mTORC2 to make a separate section.
• Maybe add a short comparison to mTORC1, but might already be covered in main mTOR article. How are the two pathways different if they both involve AKT?
• How do insulin, growth factors, serum, and nutrient levels affect mTORC2?

BreCaitlin (talk) 17:18, 1 May 2013 (UTC)

mTORC2 (and other related mTOR wiki articles).

As a reader feedback, I found these articles generally readable, accessible, and very helpful. Thank you. (I utilise wikipedia articles like this to assist my understanding of biology re my advocacy role on the disease issue of Chronic Fatigue Syndrome (CFS), ME, CFIDS, FM.)

1 - Question: Should the word "lethal" re "mammalian lethal" be in the text, 2nd paragraph:

    "mTOR Complex 1 (mTORC1) is composed of mTOR itself, regulatory-associated protein of mTOR (Raptor), mammalian lethal with SEC13 ...."

This does not seem to read right, and the meaning to me is unclear. Should it perhaps read: ... lethal for mammalian cells with ... ???

2 - A point of some confusion as reader:

Reading several Wiki and non wiki articles concerning mTOR, I found some confusion if rapamycin = mTOR, or if rapamycin activates or suppresses mTOR. That is to say they are different entities.

For example in the Wikipedia article, https://en.wikipedia.org/wiki/Sirolimus, under Lupus,

   " ... that inhibiting mTOR with rapamycin may be a disease-modifiying treatment.[54]".  

This suggest rapamycin is a different entity that regulates mTOR. Yet other articles are clear rapamycin is another name for mTOR. (And also rapamycin=sirolimus.) Perhaps, rapamycin is mTOR, and rapamycin can be given, to regulate the level of mTORC1 and mTORC2 as derivative but different active entities?

Perhaps in any further review of this and other mTOR articles, the relationships can be made clearer for a reader not in this field.

Hope this comment helps the excellent work.

CitizenofEarth001 (talk) 02:40, 9 December 2017 (UTC)