Talk:Immunoprecipitation

Great article, but what's not clear is

why you would want to do this? what is the application?

Citations

This page lacks references, or at least there are not enough. There isn't a single reference about co-immunoprecipitation, and people often need to get to the source of all the information. I wouldn't put 'citation needed' anywhere, however, because the information is true, it's just that there isn't a single source.

Co-immunoprecipitation

What about problems with co-immunoprecipitation, ie. the need for specific antibodies, the fact that you can only target already known proteins? —Preceding unsigned comment added by 72.38.153.239 (talk) 14:18, 14 April 2008 (UTC)

What is input?

Immunoblots usually show one column labelled "input". What exactly is this? — Preceding unsigned comment added by 76.105.5.61 (talk) 04:57, 16 October 2013 (UTC)

Needs balance

This article looks like the weekly emails I get from Invitrogen telling me that I can't possibly get good results using agarose beads for an IP. They are trying to push everyone to (their) magnetic beads which are more expensive and often lead to higher background. It would be nice to see a more balanced article rather than a sales pitch. —Preceding unsigned comment added by 184.216.193.83 (talk) 18:41, 15 November 2010 (UTC)

Fundamentally wrong?

I believe that this article is either fundamentally wrong, or at the very least the introductory paragraph is wrong. Classically, the term immunoprecipitation refers to using both a primary and a secondary antibody or a primary antibody and protein A to turn soluble proteins into a insoluble large complex that can be isolated by centrifugation (example see here: http://www.millipore.com/immunodetection/id3/immunoprecipitation). It seems that these days purification of a protein with antibody-coupled beads or resin is often called immunoprecipitation, even though I don't think this is historically accurate. At the very least the sentence " Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure." is most certainly false. Immunoprecipitation can be performed without coupling antibodies to a solid substrate and this is how it was most commonly performed as little as 15 years ago.Hrun0815 (talk) 03:11, 11 January 2013 (UTC)

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Assessment comment

The comment(s) below were originally left at Talk:Immunoprecipitation/Comments, and are posted here for posterity. Following several discussions in past years, these subpages are now deprecated. The comments may be irrelevant or outdated; if so, please feel free to remove this section.

The definition of the "pull-down" technique is different in this article and in the "protein-protein interaction" article; which one is correct? 83.31.120.58 (talk) 23:35, 27 December 2007 (UTC) This article is a very good for those who are new to Immunoprecipiattion as it is written is very easy, detailed and illustrative language. I must thank to the author.

Last edited at 15:48, 26 September 2009 (UTC). Substituted at 18:47, 29 April 2016 (UTC)