Talk:High-content screening

Where we are

The section titled "Where we are" (I added a capital) seems pretty argumentative and not particularly informative to boot. The entire passage boils down to: "As of this edit, HCS is a new and evolving technique whose impacts in science and industry are unclear." 66.93.3.210 13:25, 1 August 2007 (UTC)

Marketing Fluff

This article is a blatant piece of marketing fluff intended to promote sales, hardly a scholarly analysis or history. It should either be completely reworked or else deleted in its entirety.

this article

you are welcome to your opinion, but then so are the author(s). if you do not appreciate it, edit it and make it better.

where is the marketing fluff here then? I can not see what is being sold or marketed in the article. The reality is, technology is important and it is made useful by companies and not research scientists in labs.

There are plenty of 'scholarly' reviews and this is wiki not a science journal. There is not a lot of history to high content screening, unless you would prefer to be academically correct and begin with Hooke in the 17th century. if you do not appreciate this article, edit it and make it better etc.

59.10.140.13 06:21, 7 January 2007 (UTC)

I suggest removing the entire Technology Provides section. It seems to be just a laundry list of companies without other useful information. These would be better served by either an external webpage linked at the bottom of the article, or perhaps (though probably not), the list being moved to the bottom of the page to give it less "weight" in the article body. 65.96.186.162 05:19, 11 March 2007 (UTC)

Definition of HCS

I don't think the definition of High Content Screening is correct, in terms of the statement concerning more than one measurement per experiment. HCS implies large amounts of data, through numbers of cells time number of measurements per cell, etc, not simply more than one measurement per experiment. —Preceding unsigned comment added by 64.73.236.98 (talk) 16:21, 22 April 2008 (UTC)

I agree. Here's an introductory paragraph (which I wrote for another paper), which might be useful to adjust some of the content:

High Content Screening is a powerful technique for biological exploration and discovery, in which large collections of chemical compounds or gene perturbants are applied to cells. The cells are allowed to grow under the influence of these treatments, then are fixed and stained with fluorescent markers for proteins relevant to the biological experiment or analysis being performed. The cells are then imaged using an automated microscope, and the images analyzed to identify individual cells and measure their properties. This results in a large set of high-dimensional measurements of individual cells, separated into populations based on treatment (chemical or perturbant). This data is then analyzed to identify chemicals or genes that impact a particular biological pathway or process, and "hits" (treatments showing a potential effect) are explored further in validation and follow-up experiments. — Preceding unsigned comment added by 193.52.24.125 (talk) 09:13, 30 January 2012 (UTC)

References

The references at the end of the article do not link to specific parts of the text. This makes it difficult for me to actually verify what this article has said. —Preceding unsigned comment added by 141.211.182.53 (talk) 20:36, 10 June 2010 (UTC)