Talk:Chromatin immunoprecipitation

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note edit

Latest comment: 15 years ago1 comment1 person in discussion

I think a picture would really help for ChIP! anyone? Bform (talk) 02:40, 14 July 2008 (UTC)Reply

Recent Controversy - False Positives Could be a Problem edit

Latest comment: 10 years ago1 comment1 person in discussion

I think it would be worth mentioning a couple of recent papers which claim to show that this technique can have a high rate of false positives. A number of published papers may have reached invalid conclusions as a result.

More info here: http://www.yeastgenome.org/cutting-down-on-the-chips

Marchino61 (talk) 09:00, 30 January 2014 (UTC)Reply

Insertional ChIP (iChIP) edit

Latest comment: 9 years ago2 comments1 person in discussion

Discussed at PMID 25428274 PMC 4253623 and at PMID 20173072 PMC 2849220. Topical now, as used in the development of teixobactin. I'm not sure if review sources are yet available. LeadSongDog come howl! 17:56, 9 January 2015 (UTC)Reply

Needs to be distinguished from the "isolation chip (ichip)" device discussed at PMID 20173072 PMC 2849220 which is quite a different device.LeadSongDog come howl! 21:39, 9 January 2015 (UTC)Reply

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References

^ Schuch, Ronaldo; Agelopoulos, Konstantin; Neumann, Anna; Brandt, Burkhard; Bürger, Horst; Korsching, Eberhard (December 2012). "Site-specific chromatin immunoprecipitation: a selective method to individually analyze neighboring transcription factor binding sites in vivo". BMC Research Notes. 5 (1): 109. doi:10.1186/1756-0500-5-109.{{cite journal}}: CS1 maint: unflagged free DOI (link)
^ Rosenfeld, John M; Cooke, Tracy; Li, Zirong; Saito, Kan; Taganov, Konstantin; Thyagarajan, Bhaskar; Solache, Alejandra (March 2013). "Systematic optimization of parameters involved in preparation of chromatin and chromatin immunoprecipitation (ChIP) workflow". Epigenetics & Chromatin. 6 (S1): P122, 1756–8935–6-S1-P122. doi:10.1186/1756-8935-6-S1-P122.{{cite journal}}: CS1 maint: unflagged free DOI (link)

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[1] Schuch, Ronaldo; Agelopoulos, Konstantin; Neumann, Anna; Brandt, Burkhard; Bürger, Horst; Korsching, Eberhard (December 2012). "Site-specific chromatin immunoprecipitation: a selective method to individually analyze neighboring transcription factor binding sites in vivo". BMC Research Notes. 5 (1): 109. doi:10.1186/1756-0500-5-109.{{cite journal}}: CS1 maint: unflagged free DOI (link)

[2] Rosenfeld, John M; Cooke, Tracy; Li, Zirong; Saito, Kan; Taganov, Konstantin; Thyagarajan, Bhaskar; Solache, Alejandra (March 2013). "Systematic optimization of parameters involved in preparation of chromatin and chromatin immunoprecipitation (ChIP) workflow". Epigenetics & Chromatin. 6 (S1): P122, 1756–8935–6-S1-P122. doi:10.1186/1756-8935-6-S1-P122.{{cite journal}}: CS1 maint: unflagged free DOI (link)

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