In plasmids, the regulatory region of repBA gene forms a pseudoknot. The repA gene, which encodes a protein likely to function as an initiator for replication, and the repB gene are translationally coupled. The leader sequence of the repA mRNA contains two complementary sequences of 8 bases. Base-pairing between these two sequences forms a pseudoknot which is essential for translation. The first of these complementary sequences is found within a stem-loop, which forms a target for RNAI. Binding of RNAI to this stem-loop inhibits pseudoknot formation and translation of RepA.[1][2]
| Pseudoknot of the regulatory region of the repBA gene | |
|---|---|
 Secondary structure of the pseudoknot of the regulatory region of the repBA gene | |
| Identifiers | |
| Rfam | RF01089 |
| Other data | |
| RNA type | Cis-reg |
| Domain(s) | Eukaryotes Prokaryotes |
| PDB structures | PDBe |
References edit
- ^ Athanasopoulos V, Praszkier J, Pittard AJ (August 1995). "The replication of an IncL/M plasmid is subject to antisense control". J. Bacteriol. 177 (16): 4730–4741. PMC 177239. PMID 7543895.
- ^ Athanasopoulos V, Praszkier J, Pittard AJ (March 1999). "Analysis of elements involved in pseudoknot-dependent expression and regulation of the repA gene of an IncL/M plasmid". J. Bacteriol. 181 (6): 1811–1819. PMC 93579. PMID 10074073.