RBBP8

Retinoblastoma-binding protein 8 is a protein that in humans is encoded by the RBBP8 gene.

RBBP8
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2L4Z, 4D2H
Identifiers
Aliases	RBBP8, COM1, CTIP, JWDS, RIM, SAE2, SCKL2, retinoblastoma binding protein 8, RB binding protein 8, endonuclease
External IDs	OMIM: 604124; MGI: 2442995; HomoloGene: 28546; GeneCards: RBBP8; OMA:RBBP8 - orthologs
Gene location (Human) Chr. Chromosome 18 (human) Band 18q11.2 Start 22,798,261 bp End 23,026,488 bp
Gene location (Mouse) Chr. Chromosome 18 (mouse) Band 18|18 A1 Start 11,766,333 bp End 11,878,278 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Epithelium of choroid plexus gonad testicle kidney tubule caput epididymis epithelium of lactiferous gland skin of hip lactiferous duct ventricular zone islet of Langerhans Top expressed in genital tubercle tail of embryo vestibular sensory epithelium zygote urothelium medullary collecting duct primitive streak spermatocyte secondary oocyte transitional epithelium of urinary bladder More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nuclease activity hydrolase activity endonuclease activity transcription corepressor activity damaged DNA binding DNA binding protein binding single-stranded DNA endodeoxyribonuclease activity identical protein binding Y-form DNA binding double-strand/single-strand DNA junction binding double-stranded DNA binding single-stranded DNA binding flap-structured DNA binding Cellular component transcription repressor complex chromosome nucleus nucleoplasm intracellular membrane-bounded organelle site of double-strand break Biological process cell cycle DNA repair nucleotide-excision repair cell division nucleic acid phosphodiester bond hydrolysis response to estradiol meiosis regulation of transcription by RNA polymerase II cellular response to DNA damage stimulus blastocyst hatching DNA double-strand break processing DNA replication DNA double-strand break processing involved in repair via single-strand annealing negative regulation of transcription by RNA polymerase II double-strand break repair via homologous recombination negative regulation of G0 to G1 transition negative regulation of nucleic acid-templated transcription G1/S transition of mitotic cell cycle regulation of signal transduction by p53 class mediator Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5932 225182 Ensembl ENSG00000101773 ENSMUSG00000041238 UniProt Q99708 Q80YR6 RefSeq (mRNA) NM_002894 NM_203291 NM_203292 NM_001081223 NM_001252495 NM_175458 RefSeq (protein) NP_002885 NP_976036 NP_976037 NP_002885.1 NP_976036.1 NP_001074692 NP_001239424 Location (UCSC) Chr 18: 22.8 – 23.03 Mb Chr 18: 11.77 – 11.88 Mb PubMed search
Wikidata
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Function

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.

Interactions

RBBP8 has been shown to interact with:

• ATM,
• BRCA1,
• CTBP1,
• LMO4,
• RB1,
• RBL1,
• RBL2, and
• SIAH1

Further reading

• Deng CX, Brodie SG (2000). "Roles of BRCA1 and its interacting proteins". BioEssays. 22 (8): 728–37. doi:10.1002/1521-1878(200008)22:8<728::AID-BIES6>3.0.CO;2-B. PMID 10918303. S2CID 28860630.
• Matoba R, Okubo K, Hori N, Fukushima A, Matsubara K (1994). "The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression". Gene. 146 (2): 199–207. doi:10.1016/0378-1119(94)90293-3. PMID 8076819.
• Schaeper U, Subramanian T, Lim L, Boyd JM, Chinnadurai G (1998). "Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular protein is disrupted by E1A through a conserved PLDLS motif". J. Biol. Chem. 273 (15): 8549–52. doi:10.1074/jbc.273.15.8549. PMID 9535825.
• Yu X, Wu LC, Bowcock AM, Aronheim A, Baer R (1998). "The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repression". J. Biol. Chem. 273 (39): 25388–92. doi:10.1074/jbc.273.39.25388. PMID 9738006.
• Wong AK, Ormonde PA, Pero R, Chen Y, Lian L, Salada G, Berry S, Lawrence Q, Dayananth P, Ha P, Tavtigian SV, Teng DH, Bartel PL (1998). "Characterization of a carboxy-terminal BRCA1 interacting protein". Oncogene. 17 (18): 2279–85. doi:10.1038/sj.onc.1202150. PMID 9811458. S2CID 23495632.
• Li S, Chen PL, Subramanian T, Chinnadurai G, Tomlinson G, Osborne CK, Sharp ZD, Lee WH (1999). "Binding of CtIP to the BRCT repeats of BRCA1 involved in the transcription regulation of p21 is disrupted upon DNA damage". J. Biol. Chem. 274 (16): 11334–8. doi:10.1074/jbc.274.16.11334. PMID 10196224.
• Meloni AR, Smith EJ, Nevins JR (1999). "A mechanism for Rb/p130-mediated transcription repression involving recruitment of the CtBP corepressor". Proc. Natl. Acad. Sci. U.S.A. 96 (17): 9574–9. Bibcode:1999PNAS...96.9574M. doi:10.1073/pnas.96.17.9574. PMC 22250. PMID 10449734.
• Kim ST, Lim DS, Canman CE, Kastan MB (2000). "Substrate specificities and identification of putative substrates of ATM kinase family members". J. Biol. Chem. 274 (53): 37538–43. doi:10.1074/jbc.274.53.37538. PMID 10608806.
• Yu X, Baer R (2000). "Nuclear localization and cell cycle-specific expression of CtIP, a protein that associates with the BRCA1 tumor suppressor". J. Biol. Chem. 275 (24): 18541–9. doi:10.1074/jbc.M909494199. PMID 10764811.
• Dick FA, Sailhamer E, Dyson NJ (2000). "Mutagenesis of the pRB Pocket Reveals that Cell Cycle Arrest Functions Are Separable from Binding to Viral Oncoproteins". Mol. Cell. Biol. 20 (10): 3715–27. doi:10.1128/MCB.20.10.3715-3727.2000. PMC 85672. PMID 10779361.
• Fanciulli M, Bruno T, Di Padova M, De Angelis R, Iezzi S, Iacobini C, Floridi A, Passananti C (2000). "Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb". FASEB J. 14 (7): 904–12. doi:10.1096/fasebj.14.7.904. PMID 10783144. S2CID 43175069.
• Li S, Ting NS, Zheng L, Chen PL, Ziv Y, Shiloh Y, Lee EY, Lee WH (2000). "Functional link of BRCA1 and ataxia telangiectasia gene product in DNA damage response". Nature. 406 (6792): 210–5. doi:10.1038/35018134. PMID 10910365. S2CID 3266654.
• Zheng L, Pan H, Li S, Flesken-Nikitin A, Chen PL, Boyer TG, Lee WH (2000). "Sequence-specific transcriptional corepressor function for BRCA1 through a novel zinc finger protein, ZBRK1". Mol. Cell. 6 (4): 757–68. doi:10.1016/S1097-2765(00)00075-7. PMID 11090615.
• Izutsu K, Kurokawa M, Imai Y, Maki K, Mitani K, Hirai H (2001). "The corepressor CtBP interacts with Evi-1 to repress transforming growth factor beta signaling". Blood. 97 (9): 2815–22. doi:10.1182/blood.V97.9.2815. PMID 11313276.
• Melhuish TA, Gallo CM, Wotton D (2001). "TGIF2 interacts with histone deacetylase 1 and represses transcription". J. Biol. Chem. 276 (34): 32109–14. doi:10.1074/jbc.M103377200. PMID 11427533.
• Wu-Baer F, Baer R (2001). "Effect of DNA damage on a BRCA1 complex". Nature. 414 (6859): 36. doi:10.1038/35102118. PMID 11689934. S2CID 4329675.
• Sum EY, Peng B, Yu X, Chen J, Byrne J, Lindeman GJ, Visvader JE (2002). "The LIM domain protein LMO4 interacts with the cofactor CtIP and the tumor suppressor BRCA1 and inhibits BRCA1 activity". J. Biol. Chem. 277 (10): 7849–56. doi:10.1074/jbc.M110603200. PMID 11751867.
• Koipally J, Georgopoulos K (2002). "Ikaros-CtIP interactions do not require C-terminal binding protein and participate in a deacetylase-independent mode of repression". J. Biol. Chem. 277 (26): 23143–9. doi:10.1074/jbc.M202079200. PMID 11959865.