Premnaspirodiene oxygenase (EC 1.14.13.121, HPO, Hyoscymus muticus premnaspirodiene oxygenase) is an enzyme with systematic name (-)-vetispiradiene,NADPH:oxygen 2alpha-oxidoreductase.[1] This enzyme catalyses the following chemical reaction
- (-)-vetispiradiene + 2 NADPH + 2 H+ + 2 O2 solavetivone + 2 NADP+ + 3 H2O (overall reaction)
- (1a) (-)-vetispiradiene + NADPH + H+ + O2 solavetivol + NADP+ + H2O
- (1b) solavetivol + NADPH + H+ + O2 solavetivone + NADP+ + 2 H2O
Premnaspirodiene oxygenase heme-thiolate protein (P-450).