Trinity College Kirk was a royal collegiate church in Edinburgh, Scotland. The kirk and its adjacent almshouse, Trinity Hospital, were founded in 1460 by Mary of Gueldres in memory of her husband, King James II. Queen Mary was interred in the church, until her coffin was moved to Holyrood Abbey in 1848.
The church and hospital of Soutra Aisle dedicated to the Holy Trinity, was held as a prebend of the chancellor of St Andrews. In 1459/60 the chancellorship was vacant allowing the dowager queen to supplicate Pope Pius II for the annexation of Soutra to her Trinity College foundation – the sanctioning bull was published on 23 October 1460. Queen Mary issued a charter on 25 March 1462 detailing the constitution for Trinity College in which the provost was to hold Soutra church as a prebend but had to maintain three bedesmen in the Soutra hospital.
Built in the shadow of Calton Hill, the gothic kirk, and its associated hospital, were demolished in 1848, despite a formal protest from the Society of Antiquaries of Scotland, to allow for the construction of Waverley Station.