O27 may refer to:
- Fokker O-27, an observation aircraft of the United States Army Air Corps
- HNLMS O 27, a submarine of the Royal Netherlands Navy
- O27 gauge, a model railway scale
- Oakdale Airport, in Stanislaus County, California, United States
- Oxygen-27, an isotope of oxygen
This disambiguation page lists articles associated with the title O27.
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