The NhaD family (TC# 2.A.62) belongs to the Ion Transporter (IT) Superfamily.[1] A representative list of proteins belonging to the NhaD family can be found in the Transporter Classification Database.

The NhaD Na+/H+ antiporter has been characterized from two Vibrio species: V. parahaemolyticus and V. cholerae and in the haloalkaliphile, Alkalimonas amylolytica.[2][3] These proteins and their homologues are 400-500 aas long and exhibit 10-13 TMSs. They catalyze Na+/H+ and Li+/H+ antiport. They exhibit activity at basic pH (8-10) with no activity at pH 7.5. The Amylolytica antiporter has low Na+ affinity and has optimal activity at 600 mM Na+. Homologues are found in Pseudomonadota of all groups, Flavobacteriia, and Chlamydia. Distant homologues of the IT superfamily are ubiquitous.

The generalized reaction catalyzed by NhaD is:

nH+ (in) + mNa+ (out) ⇌ nH+ (out) + mNa+ (in).

See also

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Further reading

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References

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  1. ^ Prakash, Shraddha; Cooper, Garret; Singhi, Soumya; Saier, Milton H. (2003-12-03). "The ion transporter superfamily". Biochimica et Biophysica Acta (BBA) - Biomembranes. 1618 (1): 79–92. doi:10.1016/j.bbamem.2003.10.010. ISSN 0006-3002. PMID 14643936.
  2. ^ Nozaki, K.; Kuroda, T.; Mizushima, T.; Tsuchiya, T. (1998-03-02). "A new Na+/H+ antiporter, NhaD, of Vibrio parahaemolyticus". Biochimica et Biophysica Acta (BBA) - Biomembranes. 1369 (2): 213–220. doi:10.1016/s0005-2736(97)00223-x. ISSN 0006-3002. PMID 9518619.
  3. ^ Ostroumov, Elena; Dzioba, Judith; Loewen, Peter C.; Dibrov, Pavel (2002-08-19). "Asp(344) and Thr(345) are critical for cation exchange mediated by NhaD, Na(+)/H(+) antiporter of Vibrio cholerae". Biochimica et Biophysica Acta (BBA) - Biomembranes. 1564 (1): 99–106. doi:10.1016/s0005-2736(02)00407-8. ISSN 0006-3002. PMID 12101001.

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