MeT-5a (Mesothelial cells transfected with pRSV-T 5A) is an epithelial cell line from the human pleural mesothelium that was isolated from the pleural fluids of non-cancerous adult male individual and subsequently transfected with a PBR322 based plasmid containing the coding region for SV40 in order to immortalise them.
MeT-5a are used as a laboratory model for the study of the function and pathology of the pleural mesothelium and is often used as a control cell line in research into conditions such as mesothelioma.[1] MeT-5a are used due to their relatively cheap cost compared to primary models of the pleural mesothelium, as well as their hardiness, and their ability to indefinitely proliferate in a laboratory setting. MeT-5a cells were first isolated, immortalised, and cultured in the 1980s.[2]
References
edit- ^ Bonsall., Hubbard., Jithin., Anslow., Todd., Rowding., Filarowski., Duly, Wilson, Porter, Turega, Haywood-Small (5 November 2022). "Water-Soluble Truncated Fatty Acid–Porphyrin Conjugates Provide Photo-Sensitizer Activity for Photodynamic Therapy in Malignant Mesothelioma". Cancers. 14 (21): 5446. doi:10.3390/cancers14215446. PMC 9654571. PMID 36358864.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Lechner., Tokiwa., LaVeck., Benedict., Banks-Schlegel., Yeager., Banerjee., Harris. (1985). "Asbestos-associated chromosomal changes in human mesothelial cells". Proceedings of the National Academy of Sciences of the United States of America. 82 (11): 3884–3888. Bibcode:1985PNAS...82.3884L. doi:10.1073/pnas.82.11.3884. PMC 397893. PMID 2987952.
{{cite journal}}: CS1 maint: multiple names: authors list (link)