HEPBS

HEPBS (N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid)) is a zwitterionic organic chemical buffering agent; one of Good's buffers. HEPBS and HEPES have very similar structures and properties, HEPBS also having an acidity (pK_a) in the physiological range (7.6-9.0 useful range). This makes it possible to use it for cell culture work.

HEPBS

Names
Preferred IUPAC name 4-[4-(2-Hydroxyethyl)piperazin-1-yl]butane-1-sulfonic acid
Other names HEPBS
Identifiers
CAS Number	161308-36-7
3D model (JSmol)	Interactive image
ECHA InfoCard	100.157.294
PubChem CID	9965191
CompTox Dashboard (EPA)	DTXSID20433437
InChI InChI=1S/C10H22N2O4S/c13-9-8-12-6-4-11(5-7-12)3-1-2-10-17(14,15)16/h13H,1-10H2,(H,14,15,16) Key: LOJNFONOHINEFI-UHFFFAOYSA-N
SMILES C1CN(CCN1CCCCS(=O)(=O)O)CCO
Properties
Chemical formula	C10H22N2O4S
Molar mass	266.356 g/mol
Appearance	White crystalline powder
Density	1.25 g/cm3 (predicted)
Melting point	211–216 °C (412–421 °F; 484–489 K)
Acidity (pKa)	8.3
Hazards
Occupational safety and health (OHS/OSH):
Main hazards	Irritant
GHS labelling:
Signal word	Warning
Hazard statements	H315, H319, H335
Precautionary statements	P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501
Flash point	Non-flammable
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Infobox references

HEPBS ¹H NMR spectrum.