E65 may refer to:
- BMW E65/E66, a BMW car platform
- Eiffel 65, a Europop band from Italy
- European route E65, a north-south route connecting Malmö in Sweden and Chaniá in Greece
- Nokia E65, a smartphone
- An envelope size (110mm × 220mm, holds ⅓ A4), also known as DL
- Shin-Kūkō Expressway, route E65 in Japan
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