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is crucial for understanding the activities of cellular components in cell biology. Genetically encoded sensors have transformed this field and typically consist of two parts: the sensing domain, which detects cellular activity or interactions, and the reporting domain, which produces measurable signals. There are two main types of sensors: FRET-based sensors using two fluorophores for precise quantification but with some limitations, and single-fluorophore biosensors that are smaller, faster, and allow for multiplexed experiments, but may have challenges in obtaining absolute values and detecting response saturation. Various microscopy methods, including super-resolution optical fluctuation imaging, have been used to quantify and monitor biological activities in real time. Examples include calcium, pH, and voltage sensing. Greenwald et al. offer a more comprehensive overview of these applications.
References edit
- ^ Hugelier, Siewert; Colosi, P.L.; Lakadamyali, Melike (2023). "Quantitative Single-Molecule Localization Microscopy". Annual Review of Biophysics. 52: 139–160. doi:10.1146/annurev-biophys-111622-091212. PMID 37159293.