CrfA RNA (Caulobacter response to famine RNA) is a family of non-coding RNAs found in Caulobacter crescentus. CrfA is expressed upon carbon starvation and is thought to activate 27 genes.[1] It was originally identified along with 26 other non-coding RNAs using a tiled Caulobacter microarray protocol specifically aimed at detecting small RNAs.[2]

CrfA RNA
Conserved secondary structure of CrfA RNA.
Identifiers
SymbolCrfA
Other data
Domain(s)Caulobacter
PDB structuresPDBe

CrfA RNA is one of only 8 putative ncRNAs conserved in the closely related Caulobacter sp. K31.[1]

Carbon starvation response

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CrfA was found to be upregulated 10-fold in C. crescentus when glucose was deprived in a minimal medium. Further experimentation found the response to be specific to carbon deprivation; its expression was not increased when phosphate or nitrogen were limiting factors.[1]

Affymetrix microarrays were then used to analyse changes in the transcriptome in response to increased CrfA. Seven affected gene products were TonB-dependent receptors, outer membrane proteins which facilitate the uptake of external substrate.[3] The upregulation of these proteins could increase the carbon uptake during starvation. Another CrfA regulated gene (EntrezGene CC_1363) is thought to encode a proton pump powered by pyrophosphate hydrolysis. Increasing production of this protein could enable the cell to maintain its electrochemical gradient and power ATP synthesis during carbon starvation.[1]

σ54 regulates many other proteins involved in the carbon starvation response at the level of transcription.[4]

References

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  1. ^ a b c d Landt SG, Lesley JA, Britos L, Shapiro L (September 2010). "CrfA, a Small Noncoding RNA Regulator of Adaptation to Carbon Starvation in Caulobacter crescentus". J. Bacteriol. 192 (18): 4763–4775. doi:10.1128/JB.00343-10. PMC 2937403. PMID 20601471.
  2. ^ Landt SG, Abeliuk E, McGrath PT, Lesley JA, McAdams HH, Shapiro L (May 2008). "Small non-coding RNAs in Caulobacter crescentus". Mol. Microbiol. 68 (3): 600–614. doi:10.1111/j.1365-2958.2008.06172.x. PMC 7540941. PMID 18373523.
  3. ^ Koebnik R (August 2005). "TonB-dependent trans-envelope signalling: the exception or the rule?". Trends Microbiol. 13 (8): 343–347. doi:10.1016/j.tim.2005.06.005. PMID 15993072.
  4. ^ England JC, Perchuk BS, Laub MT, Gober JW (February 2010). "Global regulation of gene expression and cell differentiation in Caulobacter crescentus in response to nutrient availability". J. Bacteriol. 192 (3): 819–833. doi:10.1128/JB.01240-09. PMC 2812448. PMID 19948804.

Further reading

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