Amorpha-4,11-diene 12-monooxygenase (EC 1.14.13.158, CYP71AV1) is an enzyme with systematic name amorpha-4,11-diene,NADPH:oxygen oxidoreductase (12-hydroxylating).[1] This enzyme catalyses the following chemical reaction
| Amorpha-4,11-diene 12-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.13.158 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+ artemisinate + 3 NADP+ + 4 H2O (overall reaction)
- (1a) amorpha-4,11-diene + O2 + NADPH + H+ artemisinic alcohol + NADP+ + H2O
- (1b) artemisinic alcohol + O2 + NADPH + H+ artemisinic aldehyde + NADP+ + 2 H2O
- (1c) artemisinic aldehyde + O2 + NADPH + H+ artemisinate + NADP+ + H2O
Amorpha-4,11-diene 12-monooxygenase is a heme-thiolate protein (P-450)
References
edit- ^ Teoh KH, Polichuk DR, Reed DW, Nowak G, Covello PS (February 2006). "Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin". FEBS Letters. 580 (5): 1411–6. doi:10.1016/j.febslet.2006.01.065. PMID 16458889.
External links
edit- Amorpha-4,11-diene+12-monooxygenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
