Akuffo (also Akufo) is an Akan language patronymic surname with Akuapem-Akropong origins. Notable people with the Akan surname include:
- Edward Akufo-Addo (1906–1979), lawyer, former Chief Justice and President of Ghana in the Second Republic
- Fred Akuffo (1937–1979), Ghanaian soldier, politician, former Head of State of Ghana
- Gloria Akuffo, Ghanaian politician and current Attorney-General of Ghana
- Nana Akufo-Addo (born 1944), Ghanaian lawyer and current President of Ghana
- Ohenewa Akuffo (born 1979), Canadian sport wrestler
- Sophia Akuffo, Ghanaian lawyer, judge and Chief Justice of Ghana