Abieta-7,13-dien-18-ol hydroxylase (EC 1.14.13.109, CYP720B1, PTAO) is an enzyme with systematic name abieta-7,13-dien-18-ol,NADPH:oxygen oxidoreductase (18-hydroxylating).[1][2][3] This enzyme catalyses the following chemical reaction
| Abieta-7,13-dien-18-ol hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.13.109 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- abieta-7,13-dien-18-ol + NADPH + H+ + O2 abieta-7,13-dien-18-al + NADP+ + 2 H2O (overall reaction)
- (1a) abieta-7,13-dien-18-ol + NADPH + H+ + O2 abieta-7,13-dien-18,18-diol + + NADP+ + H2O:
- (1b) abieta-7,13-dien-18,18-diol abieta-7,13-dien-18-al + H2O (spontaneous)
Abietadienol hydroxylase is a heme-thiolate protein (P-450).
References
edit- ^ Funk C, Croteau R (January 1994). "Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis". Archives of Biochemistry and Biophysics. 308 (1): 258–66. doi:10.1006/abbi.1994.1036. PMID 8311462.
- ^ Funk C, Lewinsohn E, Vogel BS, Steele CL, Croteau R (November 1994). "Regulation of Oleoresinosis in Grand Fir (Abies grandis) (Coordinate Induction of Monoterpene and Diterpene Cyclases and Two Cytochrome P450-Dependent Diterpenoid Hydroxylases by Stem Wounding)". Plant Physiology. 106 (3): 999–1005. doi:10.1104/pp.106.3.999. PMC 159624. PMID 12232380.
- ^ Ro DK, Arimura G, Lau SY, Piers E, Bohlmann J (May 2005). "Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase". Proceedings of the National Academy of Sciences of the United States of America. 102 (22): 8060–5. Bibcode:2005PNAS..102.8060R. doi:10.1073/pnas.0500825102. PMC 1138258. PMID 15911762.
External links
edit- Abieta-7,13-dien-18-ol+hydroxylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
