4-chlorobenzoate—CoA ligase

In enzymology, a 4-chlorobenzoate—CoA ligase (EC 6.2.1.33) is an enzyme that catalyzes the chemical reaction

4-Chlorobenzoate—CoA ligase
Identifiers
EC no.6.2.1.33
CAS no.141583-20-2
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
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PMCarticles
PubMedarticles
NCBIproteins
4-chlorobenzoate + CoA + ATP 4-chlorobenzoyl-CoA + AMP + diphosphate

The 3 substrates of this enzyme are 4-chlorobenzoate, CoA, and ATP, whereas its 3 products are 4-chlorobenzoyl-CoA, AMP, and diphosphate.

This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. The systematic name of this enzyme class is 4-chlorobenzoate:CoA ligase. This enzyme participates in 2,4-dichlorobenzoate degradation. It employs one cofactor, magnesium.

Structural studies

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As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1T5D and 1T5H.

References

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  • Dunaway-Mariano D, Babbitt PC (1994). "On the origins and functions of the enzymes of the 4-chlorobenzoate to 4-hydroxybenzoate converting pathway". Biodegradation. 5 (3–4): 259–76. doi:10.1007/BF00696464. PMID 7765837. S2CID 13250105.
  • Loffler F, Muller R, Lingens F (1992). "Purification and properties of 4-halobenzoate-coenzyme A ligase from Pseudomonas sp. CBS3". Biol. Chem. Hoppe-Seyler. 373 (10): 1001–7. doi:10.1515/bchm3.1992.373.2.1001. PMID 1418673.
  • Chang KH, Liang PH, Beck W, Scholten JD, Dunaway-Mariano D (1992). "Isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS-3". Biochemistry. 31 (24): 5605–10. doi:10.1021/bi00139a025. PMID 1610806.