25-hydroxycholesterol 7alpha-hydroxylase (EC 1.14.13.100, 25-hydroxycholesterol 7alpha-monooxygenase, CYP7B1, CYP7B1 oxysterol 7alpha-hydroxylase) is an enzyme with systematic name cholest-5-ene-3beta,25-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating).[1][2][3][4] This enzyme catalyses the following chemical reaction
| 25-hydroxycholesterol 7alpha-hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.13.100 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- (1) cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O
- (2) cholest-5-ene-3beta,27-diol + NADPH + H+ + O2 cholest-5-ene-3beta,7alpha,27-triol + NADP+ + H2O
25-hydroxycholesterol 7α-hydroxylase is a heme-thiolate protein (P-450).
See also
editReferences
edit- ^ Toll A, Wikvall K, Sudjana-Sugiaman E, Kondo KH, Björkhem I (September 1994). "7 alpha hydroxylation of 25-hydroxycholesterol in liver microsomes. Evidence that the enzyme involved is different from cholesterol 7 alpha-hydroxylase". European Journal of Biochemistry. 224 (2): 309–16. doi:10.1111/j.1432-1033.1994.00309.x. PMID 7925343.
- ^ Li-Hawkins J, Lund EG, Bronson AD, Russell DW (June 2000). "Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol". The Journal of Biological Chemistry. 275 (22): 16543–9. doi:10.1074/jbc.M001810200. PMID 10748047.
- ^ Ren S, Marques D, Redford K, Hylemon PB, Gil G, Vlahcevic ZR, Pandak WM (May 2003). "Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat". Metabolism. 52 (5): 636–42. doi:10.1053/meta.2003.50106. PMID 12759897.
- ^ Russell DW (2003). "The enzymes, regulation, and genetics of bile acid synthesis". Annual Review of Biochemistry. 72: 137–74. doi:10.1146/annurev.biochem.72.121801.161712. PMID 12543708.
External links
edit- 25-hydroxycholesterol+7alpha-hydroxylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
