The following are the seeded players. Seedings are based on WTA rankings as of 5 August 2024. Rankings and points before are as of 12 August 2024.
Under the 2024 WTA Rulebook, points from all combined ATP/WTA 1000 tournaments (which include the Cincinnati Open) are required to be counted in a player's ranking.
† The player's 2023 points did not count towards her ranking on 12 August 2024 because the 2023 tournament was non-mandatory. Points from her 18th best result will be deducted instead.