Belinda Bencic and Kateřina Siniaková were the defending champions, but Bencic chose not to participate this year. Siniaková played alongside Barbora Krejčíková, but lost in the first round to Margarita Gasparyan and Andrea Hlaváčková.
Gasparyan and Hlaváčková went on to win the title, defeating María Irigoyen and Paula Kania in the final, 6–4, 6–2.
| Doubles | |
|---|---|
| 2016 J&T Banka Prague Open | |
| Champions |  Margarita Gasparyan  Andrea Hlaváčková |
| Runners-up |  María Irigoyen  Paula Kania |
| Score | 6–4, 6–2 |
| Draw | 16 |
| Seeds | 4 |
Seeds edit
Raquel Atawo / Abigail Spears (semifinals)
Margarita Gasparyan / 
Andrea Hlaváčková (champions)
Chuang Chia-jung / 
Darija Jurak (quarterfinals)
Liang Chen / 
Alicja Rosolska (first round)
Draw edit
Key edit
- Q = Qualifier
- WC = Wild card
- LL = Lucky loser
- Alt = Alternate
- SE = Special exempt
- PR = Protected ranking
- ITF = ITF entry
- JE = Junior exempt
- w/o = Walkover
- r = Retired
- d = Defaulted
- SR = Special ranking
| First round | Quarterfinals | Semifinals | Final | ||||||||||||||||||||||||
| 1 |  R Atawo A Spears
| 6 | 6 | ||||||||||||||||||||||||
 L Kichenok N Kichenok
| 1 | 2 | 1 | R Atawo A Spears
| 6 | 6 | |||||||||||||||||||||
 Y Bonaventure S-w Hsieh
| 6 | 6 | Y Bonaventure S-w Hsieh
| 1 | 4 | ||||||||||||||||||||||
| WC |  J Čepelová V Kužmová
| 3 | 4 | 1 | R Atawo A Spears
| 4 | 2 | ||||||||||||||||||||
| 3 | C-j Chuang D Jurak
| 6 | 66 | [10] | M Irigoyen P Kania
| 6 | 6 | ||||||||||||||||||||
V Dushevina L Marozava
| 1 | 78 | [6] | 3 | C-j Chuang D Jurak
| 3 | 6 | [6] | |||||||||||||||||||
| B Strýcová R Voráčová
| 4 | 65 | M Irigoyen P Kania
| 6 | 3 | [10] | |||||||||||||||||||||
| M Irigoyen P Kania
| 6 | 77 | M Irigoyen P Kania
| 4 | 2 | ||||||||||||||||||||||
 O Kalashnikova S Vogt
| 0 | 0 | 2 | M Gasparyan A Hlaváčková
| 6 | 6 | |||||||||||||||||||||
 G Dabrowski MJ Martínez Sánchez
| 6 | 6 | G Dabrowski MJ Martínez Sánchez
| 1 | 6 | [11] | |||||||||||||||||||||
 A Cornet V King
| 65 | 6 | [10] | A Cornet V King
| 6 | 3 | [9] | ||||||||||||||||||||
| 4 |  C Liang A Rosolska
| 77 | 1 | [2] | G Dabrowski MJ Martínez Sánchez
| 5 | 6 | [5] | |||||||||||||||||||
| C-w Chan K Jans-Ignacik
| 77 | 6 | 2 | M Gasparyan A Hlaváčková
| 7 | 4 | [10] | ||||||||||||||||||||
| WC | T Smitková B Štefková
| 65 | 3 | C-w Chan K Jans-Ignacik
| 6 | 4 | [7] | ||||||||||||||||||||
| B Krejčíková K Siniaková
| 4 | 6 | [4] | 2 | M Gasparyan A Hlaváčková
| 4 | 6 | [10] | |||||||||||||||||||
| 2 | M Gasparyan A Hlaváčková
| 6 | 4 | [10] | |||||||||||||||||||||||