2,5-didehydrogluconate reductase

In enzymology, a 2,5-didehydrogluconate reductase (EC 1.1.1.274) is an enzyme that catalyzes the chemical reaction

2,5-didehydrogluconate reductase
Identifiers
EC no.	1.1.1.274
CAS no.	95725-95-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

2-dehydro-D-gluconate + NADP⁺ $\rightleftharpoons$ 2,5-didehydro-D-gluconate + NADPH + H⁺

Thus, the two substrates of this enzyme are 2-dehydro-D-gluconate and NADP⁺, whereas its 3 products are 2,5-didehydro-D-gluconate, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 2-dehydro-D-gluconate:NADP⁺ 2-oxidoreductase. Other names in common use include 2,5-diketo-D-gluconate reductase, and YqhE reductase.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1VP5.

References

• Yum DY, Lee BY, Pan JG (1999). "Identification of the yqhE and yafB genes encoding two 2, 5-diketo-D-gluconate reductases in Escherichia coli". Appl. Environ. Microbiol. 65 (8): 3341–6. doi:10.1128/AEM.65.8.3341-3346.1999. PMC 91502. PMID 10427017.
• Yum DY, Lee BY, Hahm DH, Pan JG (1998). "The yiaE gene, located at 80.1 minutes on the Escherichia coli chromosome, encodes a 2-ketoaldonate reductase". J. Bacteriol. 180 (22): 5984–8. doi:10.1128/JB.180.22.5984-5988.1998. PMC 107674. PMID 9811658.
• Habrych M, Rodriguez S, Stewart JD (2002). "Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE". Biotechnol. Prog. 18 (2): 257–61. doi:10.1021/bp0101841. PMID 11934293. S2CID 23141855.