Z0, Z0 or Z0 may refer to:
- Characteristic impedance, a ratio used in electronics
- Impedance of free space, a physical constant
- Z boson, an elementary particle that mediates the weak force
- a rare rail transport modelling scale
- Roughness length, a factor used in wind speed calculations
- Z0 sex-determination system in some moth species, also referring to biological females under this system
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