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Summary
| Description |
English: Used to verify sgRNA/Cas9 complex targeting activity, and the proper integrity of the added RNA module. Direct activation: The transcription activator, VP64, is fused to the Cas9 protein, so proper targeting of the complex results in reporter gene activation. Bridged activation: VP64 is fused to PP7, which recognizes and binds a sequence in the RNA module when the RNA module is properly folded. |
| Date | |
| Source | Own work |
| Author | Krisje1988 |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 01:42, 2 March 2017 | | 2,783 × 2,292 (250 KB) | Krisje1988 | User created page with UploadWizard |
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