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Summary
| Description |
English: A scheme for the engineering of higher soluble expression. POI = protein of interest. When the POI is properly folded, the T7n portion is exposed, allowing for the binding to the T7c portion to form a fully functional T7 RNAP. This T7 RNAP can then transcribe gIII. If the POI is misfolded, then the T7 RNAP doesn’t form, leading to no gIII transcription. |
| Date | |
| Source | Own work |
| Author | Alexjli |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 03:50, 13 May 2020 | | 1,139 × 788 (44 KB) | Alexjli | Cross-wiki upload from en.wikipedia.org |
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