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Summary
| Description |
English: Example SPROX assay workflow. A lysate is treated with DMSO control or with drug. Aliquots of each treatment group are split and mixed with increasing amounts of guanidine hydrochloride. Hydrogen peroxide is added to each sample. Exposed methionine residues are oxidized. Drug-bound proteins are stabilized, so they unfold less readily and expose fewer methionines to oxidation. Oxidized/unoxidized methionine-containing peptides are quantified via LC-MS/MS and stability curves are drawn for each protein in the sample. A rightward shift in the stability curve implies that ligand-induced stabilization reduced oxidation. |
| Date | |
| Source | Own work |
| Author | Sgold626 |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 21:54, 7 December 2021 | | 3,111 × 1,167 (445 KB) | Sgold626 | Uploaded own work with UploadWizard |
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