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Summary
| Description |
English: Original figure legend: Multiple fluorescence 2PE imaging. 2PE multiple fluorescence image from a 16 μm cryostat section of mouse intestine stained with a combination of fluorescent stains (F-24631, Molecular Probes). Alexa Fluor 350 wheat germ agglutinin, a blue-fluorescent lectin, was used to stain the mucus of goblet cells. The filamentous actin prevalent in the brush border was stained with red-fluorescent Alexa Flu or 568 phalloidin. Finally, the nuclei were stained with SYTOX ® Green nucleic acid stain. Imaging has been performed at 780 nm, 100 x 1.4 NA Leica objective, using a Chameleon XR ultrafast Ti-Sapphire laser (Coherent Inc., USA) coupled at LAMBS-MicroScoBio with a Spectral Confocal Laser Scanning Microscope, Leica SP2-AOBS.
Deutsch: Zweiphotonenaufnahme an einem Schnitt durch einen Mausdarm. Zellkerne in grün, Schleim der Becherzellen in blau, Aktin (Phalloidin-Färbung) in rot. Anregung erfolgte bei 780 nm durch einen Titan:Saphir-Laser. |
| Date | |
| Source |
Multi-photon excitation microscopy. BioMedical Engineering OnLine, 2006, 5:36. |
| Author | Alberto Diaspro, Paolo Bianchini, Giuseppe Vicidomini, Mario Faretta, Paola Ramoino and Cesare Usai |
| Permission (Reusing this file) |
  This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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| current | 18:59, 23 December 2008 | | 2,133 × 2,133 (949 KB) | Dietzel65 | == Beschreibung == {{Information |Description={{en|1=Original figure legend: ''Multiple fluorescence 2PE imaging. 2PE multiple fluorescence image from a 16 μm cryostat section of mouse intestine stained with a combination of fluorescent stains (F-24631, |
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