File:His-tag.png

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Summary

Description
English: Adding polyhistidine tags. A) The His-tag is added by inserting the DNA coding a protein of interest in a vector that has the tag ready to fuse at the C-terminal. B)The His-tag is added using primers containing the tag, after a PCR reaction the tag gets fused to the N-terminal of the gene. AAAsBiophysics 20:29, 17 July 2007 (UTC)
Date 17 July 2007 (original upload date)
Source Transferred from en.wikipedia to Commons by IngerAlHaosului using CommonsHelper.
Author The original uploader was Jfhaller at English Wikipedia.
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The original description page was here. All following user names refer to en.wikipedia.
  • 2007-07-17 19:18 Jfhaller 773×607× (31177 bytes) Adding polyhistidine tags. A) The His-tag is added by inserting the DNA coding a protein of interest in a vector that has the tag ready to fuse at the C-terminal. B)The His-tag is added using primers containing the tag, after a PCR reaction the tag gets f

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17 July 2007

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Date/TimeThumbnailDimensionsUserComment
current19:21, 20 January 2010Thumbnail for version as of 19:21, 20 January 2010773 × 607 (30 KB)File Upload Bot (Magnus Manske) {{BotMoveToCommons|en.wikipedia|year={{subst:CURRENTYEAR}}|month={{subst:CURRENTMONTHNAME}}|day={{subst:CURRENTDAY}}}} {{Information |Description={{en|Adding polyhistidine tags. A) The His-tag is added by inserting the DNA coding a protein of interest in
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