Cholecalciferol, also known as vitamin D3 and colecalciferol, is a type of vitamin D that is made by the skin when exposed to UV-B light; it is found in some foods and can be taken as a dietary supplement.[3]
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Pronunciation | /ˌkoʊləkælˈsɪfərɒl/ |
Other names | vitamin D3, calciol, activated 7-dehydrocholesterol |
AHFS/Drugs.com | Professional Drug Facts |
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Routes of administration | By mouth, intramuscular |
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ECHA InfoCard | 100.000.612 |
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Formula | C27H44O |
Molar mass | 384.648 g·mol−1 |
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Melting point | 83 to 86 °C (181 to 187 °F) |
Boiling point | 496.4 °C (925.5 °F) |
Solubility in water | Practically insoluble in water, freely soluble in ethanol, methanol and some other organic solvents. Slightly soluble in vegetable oils. |
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Cholecalciferol is made in the skin following UVB light exposure.[4] It is converted in the liver to calcifediol (25-hydroxyvitamin D) which is then converted in the kidney to calcitriol (1,25-dihydroxyvitamin D).[4] One of its actions is to increase calcium uptake by the intestines.[5] It is found in food such as some fish, beef liver, eggs, and cheese.[6][7] Plants, cow milk, fruit juice, yogurt, and margarine also may have cholecalciferol added to them in some countries, including the United States.[6][7]
Cholecalciferol can be taken as an oral dietary supplement to prevent vitamin D deficiency or as a medication to treat associated diseases, including rickets.[8][9] It is also used for familial hypophosphatemia, hypoparathyroidism that is causing low blood calcium, and Fanconi syndrome.[9][10] Vitamin-D supplements may not be effective in people with severe kidney disease.[11][10] Excessive doses in humans can result in vomiting, constipation, weakness, and confusion.[5] Other risks include kidney stones.[11] Doses greater than 40000 IU (1000 μg) per day are generally required before high blood calcium occurs.[12] Normal doses, 800–2000 IU per day, are safe in pregnancy.[5]
Cholecalciferol was first described in 1936.[13] It is on the World Health Organization's List of Essential Medicines.[14] In 2021, it was the 65th most commonly prescribed medication in the United States, with more than 10 million prescriptions.[15][16] Cholecalciferol is available as a generic medication and over the counter.[10][17][18]
Medical uses
editCholecalciferol (vitamin D3) appears to stimulate the body's interferon type I signaling system that protects against bacteria and viruses, unlike vitamin D2.[19]
For psoriasis, cream that includes vitamin D3 can have a good effect, or UVB phototherapy.
Vitamin D deficiency
editCholecalciferol is a form of vitamin D which is naturally synthesized in skin and functions as a pro-hormone, being converted to calcitriol. This is important for maintaining calcium levels and promoting bone health and development.[4] As a medication, cholecalciferol may be taken as a dietary supplement to prevent or to treat vitamin D deficiency. One gram is 40000000 (40×106) IU, equivalently 1 IU is 0.025 μg, or 25 ng. Dietary reference intake values for vitamin D (ergocalciferol, which is D2, or cholecalciferol, which is D3), or both, have been established and recommendations vary depending on the country:
- In the US: 15 μg/d (600 IU/d) for all individuals (males, females, pregnant/lactating women) between the ages of 1 and 70 years, inclusive. For all individuals older than 70 years, 20 μg/d (800 IU/d) is recommended.[20]
- In the EU: 15 μg/d (600 IU/d) for all people older than 1 year and 10 μg/d (400 IU/d) for infants aged 7–11 months, assuming minimal cutaneous vitamin D synthesis.[21]
- In the UK: a ‘Safe Intake’ (SI) of 8.5–10 μg/d (30–400 IU/d) for infants < 1 year (including exclusively breastfed infants) and an SI of 10 μg/d (400 IU/d) for children aged 1 to <4 years; for all other population groups aged 4 years and more (including pregnant/lactating women) a Reference Nutrient Intake (RNI) of day10 μg (400 IU/d).[22]
Low levels of vitamin D3 are more commonly found in individuals living in northern latitudes or with other reasons for a lack of regular sun exposure, including being housebound, frail, elderly, or obese, having darker skin, and wearing clothes that cover most of the skin.[23][24] Supplements are recommended for these groups of people.[24]
The Institute of Medicine in 2010 recommended a maximum uptake of vitamin D of 4000 IU/d, finding that the dose for lowest observed adverse effect level is 40,000 IU daily for at least 12 weeks,[25] and that there was a single case of toxicity above 10000 IU after more than seven years of daily intake; this case of toxicity occurred in circumstances that have led other researchers to dispute whether it is a credible case to consider when making vitamin D intake recommendations.[25] Patients with severe vitamin D deficiency will require treatment with a loading dose; its magnitude can be calculated based on the actual serum 25-hydroxy-vitamin D level and body weight.[26]
There are conflicting reports concerning the relative effectiveness of cholecalciferol (D3) versus ergocalciferol (D2), with some studies suggesting less efficacy of D2, and others showing no difference. There are differences in absorption, binding and inactivation of the two forms, with evidence usually favoring cholecalciferol in raising levels in blood, although more research is needed.[27]
A much less common use of cholecalciferol therapy in rickets utilizes a single large dose and has been called stoss therapy.[28][29][30] Treatment is given either orally or by intramuscular injection of 300000 IU (7500 μg) to 500000 IU (12500 μg = 12.5 mg), in a single dose, or sometimes in two to four divided doses. There are concerns about the safety of such large doses.[30]
Low circulating vitamin D levels have been associated with lower total testosterone levels in males. Vitamin D supplementation could potentially improve total testosterone concentration, although more research is needed.[31]
Other diseases
editA meta-analysis of 2007 concluded that daily intake of 1000 to 2000 IU/d of vitamin D3 could reduce the incidence of colorectal cancer with minimal risk.[32] Also a 2008 study published in Cancer Research has shown the addition of vitamin D3 (along with calcium) to the diet of some mice fed a regimen similar in nutritional content to a new Western diet with 1000 IU cholecalciferol per day prevented colon cancer development.[33] In humans, with 400 IU daily, there was no effect of cholecalciferol supplements on the risk of colorectal cancer.[34]
Supplements are not recommended for prevention of cancer as any effects of cholecalciferol are very small.[35] Although correlations exist between low levels of blood serum cholecalciferol and higher rates of various cancers, multiple sclerosis, tuberculosis, heart disease, and diabetes,[36] the consensus is that supplementing levels is not beneficial.[37] It is thought that tuberculosis may result in lower levels.[38] It, however, is not entirely clear how the two are related.[39]
Biochemistry
editStructure
editCholecalciferol is one of the five forms of vitamin D.[40] Cholecalciferol is a secosteroid, that is, a steroid molecule with one ring open.[41]
Mechanism of action
editBy itself cholecalciferol is inactive. It is converted to its active form by two hydroxylations: the first in the liver, by CYP2R1 or CYP27A1, to form 25-hydroxycholecalciferol (calcifediol, 25-OH vitamin D3). The second hydroxylation occurs mainly in the kidney through the action of CYP27B1 to convert 25-OH vitamin D3 into 1,25-dihydroxycholecalciferol (calcitriol, 1,25-(OH)2vitamin D3). All these metabolites are bound in blood to the vitamin D-binding protein. The action of calcitriol is mediated by the vitamin D receptor, a nuclear receptor which regulates the synthesis of hundreds of proteins and is present in virtually every cell in the body.[4]
Biosynthesis
editClick on icon in lower right corner to open.
Click on genes, proteins and metabolites below to link to respective articles. [§ 1]
- ^ The interactive pathway map can be edited at WikiPathways: "VitaminDSynthesis_WP1531".
7-Dehydrocholesterol is the precursor of cholecalciferol.[4] Within the epidermal layer of skin, 7-dehydrocholesterol undergoes an electrocyclic reaction as a result of UVB light at wavelengths between 290 and 310 nm, with peak synthesis occurring at 293 nm.[42] This results in the opening of the vitamin precursor B-ring through a conrotatory pathway making previtamin D3 (pre-cholecalciferol).[43] In a process which is independent of UV light, the pre-cholecalciferol then undergoes a [1,7] antarafacial sigmatropic rearrangement [44] and therein finally isomerizes to form vitamin D3.
The active UVB wavelengths are little present in sunlight, and sufficient amounts of cholecalciferol can be produced with moderate exposure of the skin, depending on the strength of the sun.[42] Time of day, season, latitude, and altitude affect the strength of the sun, and pollution, cloud cover or glass all reduce the amount of UVB exposure. Exposure of face, arms and legs, averaging 5–30 minutes twice per week, may be sufficient, but the darker the skin, and the weaker the sunlight, the more minutes of exposure are needed. Vitamin D overdose is impossible from UV exposure; the skin reaches an equilibrium where the vitamin degrades as fast as it is created.[42]
Cholecalciferol can be produced in skin from the light emitted by the UV lamps in tanning beds, which produce ultraviolet primarily in the UVA spectrum, but typically produce 4% to 10% of the total UV emissions as UVB. Levels in blood are higher in frequent users of tanning salons.[42]
A 293 nanometer UVB light emitting diode (LED) was found to be 2.4 times more efficient in producing vitamin D3 than the sun in less than 1⁄60 the time. (https://pubmed.ncbi.nlm.nih.gov/28904394/).
Whether cholecalciferol and all forms of vitamin D are by definition "vitamins" can be disputed, since the definition of vitamins includes that the substance cannot be synthesized by the body and must be ingested. Cholecalciferol is synthesized by the body during UVB radiation exposure.[4]
The three steps in the synthesis and activation of vitamin D3 are regulated as follows:
- Cholecalciferol is synthesized in the skin from 7-dehydrocholesterol under the action of ultraviolet B (UVB) light. It reaches an equilibrium after several minutes depending on the intensity of the UVB in the sunlight – determined by latitude, season, cloud cover, and altitude – and the age and degree of pigmentation of the skin.
- Hydroxylation in the endoplasmic reticulum of liver hepatocytes of cholecalciferol to calcifediol (25-hydroxycholecalciferol) by 25-hydroxylase is loosely regulated, if at all, and blood levels of this molecule largely reflect the amount of cholecalciferol produced in the skin combined with any vitamin D2 or D3 ingested.
- Hydroxylation in the kidneys of calcifediol to calcitriol by 1-alpha-hydroxylase is tightly regulated: it is stimulated by parathyroid hormone and serves as the major control point in the production of the active circulating hormone calcitriol (1,25-dihydroxyvitamin D3).[4]
Industrial production
editCholecalciferol is produced industrially for use in vitamin supplements and to fortify foods. As a pharmaceutical drug it is called cholecalciferol (USAN) or colecalciferol (INN, BAN). It is produced by the ultraviolet irradiation of 7-dehydrocholesterol extracted from lanolin found in sheep's wool.[45] Cholesterol is extracted from wool grease and wool wax alcohols obtained from the cleaning of wool after shearing. The cholesterol undergoes a four-step process to make 7-dehydrocholesterol, the same compound that is produced in the skin of animals. The 7-dehydrocholesterol is then irradiated with ultraviolet light. Some unwanted isomers are formed during irradiation: these are removed by various techniques, leaving a resin which melts at about room temperature and usually has a potency of 25000000 to 30000000 International Units/gram.
Cholecalciferol is also produced industrially for use in vitamin supplements from lichens, which is suitable for vegans.[46][47]
Stability
editCholecalciferol is very sensitive to UV radiation and will rapidly, but reversibly, break down to form supra-sterols, which can further irreversibly convert to ergosterol.[citation needed]
Pesticide
editRodents are somewhat more susceptible to high doses than other species, and cholecalciferol has been used in poison bait for the control of these pests.[48][18]
The mechanism of high dose cholecalciferol is that it can produce "hypercalcemia, which results in systemic calcification of soft tissue, leading to kidney failure, cardiac abnormalities, hypertension, CNS depression, and GI upset. Signs generally develop within 18–36 h of ingestion and can include depression, loss of appetite, polyuria, and polydipsia."[17] High-dose cholecalciferol will tend to rapidly accumulate in adipose tissue yet release more slowly[49] which will tend to delay time of death for several days from the time that high-dose bait is introduced.[48]
In New Zealand, possums have become a significant pest animal. For possum control, cholecalciferol has been used as the active ingredient in lethal baits.[50] The LD50 is 16.8 mg/kg, but only 9.8 mg/kg if calcium carbonate is added to the bait.[51][52] Kidneys and heart are target organs.[53] LD50 of 4.4 mg/kg has been reported in rabbits, with lethality to almost all rabbits ingesting doses greater than 15 mg/kg.[54] Toxicity has been reported across a wide range of cholecalciferol dosages, with LD50 as high as 88 mg/kg or LDLo as low as 2 mg/kg reported for dogs.[55]
Researchers have reported that the compound is less toxic to non-target species than earlier generations of anticoagulant rodenticides (Warfarin and congeners) or Bromethalin,[56] and that relay toxicosis (poisoning by eating a poisoned animal) has not been documented.[17] Nevertheless, the same source reports that use of cholecalciferol in rodenticides may still pose a significant hazard to other animals, such as dogs and cats, when rodenticide bait or other forms of cholecalciferol are directly ingested.[17]
See also
edit- Hypervitaminosis D, Vitamin D poisoning
- Ergocalciferol, vitamin D2
- 25-Hydroxyvitamin D 1-alpha-hydroxylase, a kidney enzyme that converts calcifediol to calcitriol
References
edit- ^ "Health product highlights 2021: Annexes of products approved in 2021". Health Canada. 3 August 2022. Retrieved 25 March 2024.
- ^ "Regulatory Decision Summary for Vitamin D3 Oral Solution". Health Canada. 5 February 2021. Retrieved 25 March 2024.
- ^ Coulston AM, Boushey C, Ferruzzi M (2013). Nutrition in the Prevention and Treatment of Disease. Academic Press. p. 818. ISBN 9780123918840. Archived from the original on 30 December 2016. Retrieved 29 December 2016.
- ^ a b c d e f g Norman AW (August 2008). "From vitamin D to hormone D: fundamentals of the vitamin D endocrine system essential for good health". The American Journal of Clinical Nutrition. 88 (2): 491S–499S. doi:10.1093/ajcn/88.2.491S. PMID 18689389.
- ^ a b c "Cholecalciferol (Professional Patient Advice) - Drugs.com". www.drugs.com. Archived from the original on 30 December 2016. Retrieved 29 December 2016.
- ^ a b "Office of Dietary Supplements - Vitamin D". ods.od.nih.gov. 11 February 2016. Archived from the original on 31 December 2016. Retrieved 30 December 2016.
- ^ a b Ross AC, Taylor CL, Yaktine AL, Del Valle HB, et al. (Institute of Medicine (US); Committee to Review Dietary Reference Intakes for Vitamin D and Calcium) (2011). Dietary Reference Intakes for Calcium and Vitamin D (PDF). National Academies Press. doi:10.17226/13050. ISBN 978-0-309-16394-1. PMID 21796828. S2CID 58721779.
- ^ British national formulary : BNF 69 (69 ed.). British Medical Association. 2015. pp. 703–704. ISBN 9780857111562.
- ^ a b World Health Organization (2009). Stuart MC, Kouimtzi M, Hill SR (eds.). WHO Model Formulary 2008. World Health Organization. hdl:10665/44053. ISBN 9789241547659.
- ^ a b c Hamilton R (2015). Tarascon Pocket Pharmacopoeia 2015 Deluxe Lab-Coat Edition. Jones & Bartlett Learning. p. 231. ISBN 9781284057560.
- ^ a b "Aviticol 1 000 IU Capsules - Summary of Product Characteristics (SPC) - (eMC)". www.medicines.org.uk. Archived from the original on 30 December 2016. Retrieved 29 December 2016.
- ^ Vieth R (May 1999). "Vitamin D supplementation, 25-hydroxyvitamin D concentrations, and safety" (PDF). The American Journal of Clinical Nutrition. 69 (5): 842–56. doi:10.1093/ajcn/69.5.842. PMID 10232622.
- ^ Fischer J, Ganellin CR (2006). Analogue-based Drug Discovery. John Wiley & Sons. p. 451. ISBN 978-3-527-60749-5. Archived from the original on 30 December 2016. Retrieved 29 December 2016.
- ^ World Health Organization (2019). World Health Organization model list of essential medicines: 21st list 2019. Geneva: World Health Organization. hdl:10665/325771. WHO/MVP/EMP/IAU/2019.06. License: CC BY-NC-SA 3.0 IGO.
- ^ "The Top 300 of 2021". ClinCalc. Retrieved 14 January 2024.
- ^ "Cholecalciferol - Drug Usage Statistics". ClinCalc. Retrieved 14 January 2024.
- ^ a b c d Khan SA, Schell MM (November 2014). "Merck Veterinary Manual - Rodenticide Poisoning: Introduction". Retrieved 10 October 2021.
Incidence of vitamin D3 toxicosis in animals is relatively less than that of anticoagulant and bromethalin toxicosis. Relay toxicosis from vitamin D3 has not been documented.
- ^ a b Rizor SE, Arjo WM, Bulkin S, Nolte DL. Efficacy of Cholecalciferol Baits for Pocket Gopher Control and Possible Effects on Non-Target Rodents in Pacific Northwest Forests. Vertebrate Pest Conference (2006). USDA. Archived from the original on 14 September 2012. Retrieved 27 August 2019.
0.15% cholecalciferol bait appears to have application for pocket gopher control.' Cholecalciferol can be a single high-dose toxicant or a cumulative multiple low-dose toxicant.
- ^ Haridy R (28 February 2022). "One type of vitamin D found to boost immune system, another may hinder it". New Atlas. Retrieved 7 April 2022.
- ^ DRIs for Calcium and Vitamin D Archived 24 December 2010 at the Wayback Machine
- ^ "Dietary reference values for vitamin D | EFSA". 28 October 2016.
- ^ "Joint explanatory note by the European Food Safety Authority and the UK Scientific Advisory Committee on Nutrition regarding dietary reference values for vitamin D" (PDF).
- ^ Mithal A, Wahl DA, Bonjour JP, Burckhardt P, Dawson-Hughes B, Eisman JA, El-Hajj Fuleihan G, Josse RG, Lips P, Morales-Torres J (November 2009). "Global vitamin D status and determinants of hypovitaminosis D". Osteoporos Int. 20 (11): 1807–20. doi:10.1007/s00198-009-0954-6. PMID 19543765. S2CID 52858668.
- ^ a b "Vitamins and minerals – Vitamin D". National Health Service. 3 August 2020. Retrieved 15 November 2020.
- ^ a b Vieth R (May 1999). "Vitamin D supplementation, 25-hydroxyvitamin D concentrations, and safety". The American Journal of Clinical Nutrition. 69 (5): 842–56. doi:10.1093/ajcn/69.5.842. PMID 10232622.
- ^ van Groningen L, Opdenoordt S, van Sorge A, Telting D, Giesen A, de Boer H (April 2010). "Cholecalciferol loading dose guideline for vitamin D-deficient adults". European Journal of Endocrinology. 162 (4): 805–11. doi:10.1530/EJE-09-0932. PMID 20139241.
- ^ Tripkovic L, Lambert H, Hart K, Smith CP, Bucca G, Penson S, et al. (June 2012). "Comparison of vitamin D2 and vitamin D3 supplementation in raising serum 25-hydroxyvitamin D status: a systematic review and meta-analysis". The American Journal of Clinical Nutrition. 95 (6): 1357–64. doi:10.3945/ajcn.111.031070. PMC 3349454. PMID 22552031.
- ^ Shah BR, Finberg L (September 1994). "Single-day therapy for nutritional vitamin D-deficiency rickets: a preferred method". The Journal of Pediatrics. 125 (3): 487–90. doi:10.1016/S0022-3476(05)83303-7. PMID 8071764.
- ^ Chatterjee D, Swamy MK, Gupta V, Sharma V, Sharma A, Chatterjee K (March 2017). "Safety and Efficacy of Stosstherapy in Nutritional Rickets". Journal of Clinical Research in Pediatric Endocrinology. 9 (1): 63–69. doi:10.4274/jcrpe.3557. PMC 5363167. PMID 27550890.
- ^ a b Bothra M, Gupta N, Jain V (June 2016). "Effect of intramuscular cholecalciferol megadose in children with nutritional rickets". Journal of Pediatric Endocrinology & Metabolism. 29 (6): 687–92. doi:10.1515/jpem-2015-0031. PMID 26913455. S2CID 40611968.
- ^ Chen C, Zhai H, Cheng J, Weng P, Chen Y, Li Q, et al. (August 2019). "Causal Link Between Vitamin D and Total Testosterone in Men: A Mendelian Randomization Analysis". The Journal of Clinical Endocrinology and Metabolism. 104 (8): 3148–3156. doi:10.1210/jc.2018-01874. PMID 30896763. S2CID 84841517.
- ^ Gorham ED, Garland CF, Garland FC, Grant WB, Mohr SB, Lipkin M, et al. (March 2007). "Optimal vitamin D status for colorectal cancer prevention: a quantitative meta analysis". American Journal of Preventive Medicine (Meta-Analysis). 32 (3): 210–6. doi:10.1016/j.amepre.2006.11.004. PMID 17296473.
- ^ Yang K, Kurihara N, Fan K, Newmark H, Rigas B, Bancroft L, et al. (October 2008). "Dietary induction of colonic tumors in a mouse model of sporadic colon cancer". Cancer Research. 68 (19): 7803–10. doi:10.1158/0008-5472.CAN-08-1209. PMID 18829535.
- ^ Wactawski-Wende J, Kotchen JM, Anderson GL, Assaf AR, Brunner RL, O'Sullivan MJ, et al. (February 2006). "Calcium plus vitamin D supplementation and the risk of colorectal cancer". The New England Journal of Medicine. 354 (7): 684–96. doi:10.1056/NEJMoa055222. PMID 16481636. S2CID 20826870.
- ^ Bjelakovic G, Gluud LL, Nikolova D, Whitfield K, Wetterslev J, Simonetti RG, et al. (January 2014). "Vitamin D supplementation for prevention of mortality in adults". The Cochrane Database of Systematic Reviews. 1 (1): CD007470. doi:10.1002/14651858.cd007470.pub3. PMC 11285307. PMID 24414552.
- ^ Garland CF, Garland FC, Gorham ED, Lipkin M, Newmark H, Mohr SB, Holick MF (February 2006). "The role of vitamin D in cancer prevention". American Journal of Public Health. 96 (2): 252–61. doi:10.2105/AJPH.2004.045260. PMC 1470481. PMID 16380576.
- ^ Ross AC, Manson JE, Abrams SA, Aloia JF, Brannon PM, Clinton SK, et al. (January 2011). "The 2011 report on dietary reference intakes for calcium and vitamin D from the Institute of Medicine: what clinicians need to know". The Journal of Clinical Endocrinology and Metabolism. 96 (1): 53–8. doi:10.1210/jc.2010-2704. PMC 3046611. PMID 21118827.
- ^ Gou X, Pan L, Tang F, Gao H, Xiao D (August 2018). "The association between vitamin D status and tuberculosis in children: A meta-analysis". Medicine. 97 (35): e12179. doi:10.1097/MD.0000000000012179. PMC 6392646. PMID 30170465.
- ^ Keflie TS, Nölle N, Lambert C, Nohr D, Biesalski HK (October 2015). "Vitamin D deficiencies among tuberculosis patients in Africa: A systematic review". Nutrition. 31 (10): 1204–12. doi:10.1016/j.nut.2015.05.003. PMID 26333888.
- ^ "cholecalciferol" at Dorland's Medical Dictionary
- ^ "About Vitamin D". University of California, Riverside. November 2011. Archived from the original on 16 October 2017. Retrieved 15 October 2017.
- ^ a b c d Wacker M, Holick MF (January 2013). "Sunlight and Vitamin D: A global perspective for health". Dermato-Endocrinology. 5 (1): 51–108. doi:10.4161/derm.24494. PMC 3897598. PMID 24494042.
- ^ MacLaughlin JA, Anderson RR, Holick MF (May 1982). "Spectral character of sunlight modulates photosynthesis of previtamin D3 and its photoisomers in human skin". Science. 216 (4549): 1001–3. Bibcode:1982Sci...216.1001M. doi:10.1126/science.6281884. PMID 6281884. S2CID 23011680.
- ^ Okamura WH, Elnagar HY, Ruther M, Dobreff S (1993). "Thermal [1,7]-sigmatropic shift of previtamin D3 to vitamin D3: synthesis and study of pentadeuterio derivatives". Journal of Organic Chemistry. 58 (3): 600–610. doi:10.1021/jo00055a011.
- ^ Vitamin D3 Story. Archived 22 January 2012 at the Wayback Machine Retrieved 8 April 2012.
- ^ "Vitashine Vegan Vitamin D3 Supplements". Archived from the original on 4 March 2013. Retrieved 15 March 2013.
- ^ Wang T, Bengtsson G, Kärnefelt I, Björn LO (September 2001). "Provitamins and vitamins D2and D3in Cladina spp. over a latitudinal gradient: possible correlation with UV levels". Journal of Photochemistry and Photobiology B: Biology. 62 (1–2): 118–22. doi:10.1016/s1011-1344(01)00160-9. PMID 11693362. Archived from the original on 28 October 2012.
- ^ a b CHOLECALCIFEROL: A UNIQUE TOXICANT FOR RODENT CONTROL. Proceedings of the Eleventh Vertebrate Pest Conference (1984). University of Nebraska Lincoln. March 1984. Archived from the original on 27 August 2019. Retrieved 27 August 2019.
Cholecalciferol is an acute (single-feeding) and/or chronic (multiple-feeding) rodenticide toxicant with unique activity for controlling commensal rodents including anticoagulant-resistant rats. Cholecalciferol differs from conventional acute rodenticides in that no bait shyness is associated with consumption and time to death is delayed, with first dead rodents appearing 3–4 days after treatment.
- ^ Brouwer DA, van Beek J, Ferwerda H, Brugman AM, van der Klis FR, van der Heiden HJ, Muskiet FA (June 1998). "Rat adipose tissue rapidly accumulates and slowly releases an orally-administered high vitamin D dose". The British Journal of Nutrition. 79 (6): 527–532. doi:10.1079/BJN19980091. PMID 9771340.
We investigated the effect of oral high-dose cholecalciferol on plasma and adipose tissue cholecalciferol and its subsequent release, and on plasma 25-hydroxyvitamin D (25(OH)D). ... We conclude that orally-administered cholecalciferol rapidly accumulates in adipose tissue and that it is very slowly released while there is energy balance.
- ^ "Pestoff DECAL Possum Bait - Rentokil Initial Safety Data Sheets" (PDF). Archived from the original (PDF) on 15 January 2021. Retrieved 10 May 2020.
- ^ Morgan D (2006). "Field efficacy of cholecalciferol gel baits for possum (Trichosurus vulpecula) control". New Zealand Journal of Zoology. 33 (3): 221–8. doi:10.1080/03014223.2006.9518449. S2CID 83765759.
- ^ Jolly SE, Henderson RJ, Frampton C, Eason CT (1995). "Cholecalciferol Toxicity and Its Enhancement by Calcium Carbonate in the Common Brushtail Possum". Wildlife Research. 22 (5): 579–83. doi:10.1071/WR9950579.
- ^ "Kiwicare Material Safety Data Sheet" (PDF). Archived from the original (PDF) on 10 February 2013.
- ^ R. J. Henderson and C. T. Eason (2000), Acute toxicity of cholecalciferol and gliftor baits to the European rabbit, Oryctolagus cuniculus, Wildlife Research 27(3) 297-300.
- ^ Michael E.Peterson & Kerstin Fluegeman, Cholecalciferol (Topic Review), Topics in Companion Animal Medicine, Volume 28, Issue 1, February 2013, Pages 24-27.
- ^ Kocher DK, Kaur G, Banga HS, Brar RS (2010). "Histopathological Changes in Vital Organs of House Rats Given Lethal Dose of Cholecalciferol (Vitamin D3)". Indian Journal of Animal Research. 2 (3): 193–6. ISSN 0367-6722.
Use of cholecalciferol as a rodenticide in bait lowered the risk of secondary poisoning and minimized the toxicity of non-target species